由自我DNA诱导的DNA脱甲基化激活了收获后类中模式触发的免疫力
Chunhong Li1,2, Kaituo Wang1,2, Yanyu Zou1,2,3
1Institute of Fruit Function and Disease Management, Department of Public Health and Management, Chongqing Three Gorges Medical College, Chongqing 404000, P.R. China.
Journal of experimental botany
|February 8, 2026
概括
细胞外自我DNA (sDNA) 通过降低DNA甲基化来触发植物免疫力,增强对真菌病原体的防御能力. 这种表观遗传机制涉及特定的基因和信号通路,提高果的耐药性.
科学领域:
- 植物免疫力 植物免疫力
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子植物病理学
背景情况:
- 细胞外自身DNA (sDNA) 在植物中作为损伤相关分子模式 (DAMP) 起作用.
- sDNA识别和随后的模式触发免疫 (PTI) 启动的精确机制尚未完全理解.
研究的目的:
- 阐明sDNA在果免疫中的作用.
- 研究DNA甲基化和表观遗传修饰在sDNA介导的防御反应中的参与.
主要方法:
- 用sDNA和真菌病原体 (Penicillium digitatum) 处理类水果.
- 对差异甲基化区域 (DMRs) 的DNA甲基化概况和分析.
- 对与防御相关的基因的基因表达分析,包括LRR-RLK CsSOBIR1和脱甲基酶.
- 评估植物免疫信号通路 (JA信号和CsMAPK级联).
- 用DNA甲基化抑制剂 (5-阿扎西提丁) 治疗.
主要成果:
- sDNA治疗诱导了PTI,依赖莉酸 (JA) 的防御,并降低了对P. digitatum的敏感性.
- sDNA显著降低了全基因组DNA甲基化,DMRs富含植物病原体相互作用基因.
- sDNA降低了CsSOBIR1的DNA甲基化,保持其表达并通过CsRLP7.7促进sDNA识别.
- sDNA激活了CsMAPKKK1-CsMAPKK2-CsMAPK4级联和JA信号,与去甲基酶基因的上调相关.
- 抑制DNA甲基化增强了类植物的耐药性,将脱甲基化与免疫反应相关联.
结论:
- 基因脱甲基化是类水果中sDNA介导信号传递的关键表观遗传组成部分.
- 通过CsSOBIR1和CsRLP7识别sDNA可以增强PTI和JA-依赖的防御.
- 通过DNA去甲基化进行表观遗传调节,可以增强植物对真菌病原体的免疫力.
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