相关实验视频
Updated: Feb 10, 2026

12:10
Retzius-Sparing Robot-Assisted Radical Prostatectomy
Published on: May 19, 2022
9.4K
在机器人辅助前列腺切除术中对共聚焦显微镜的可行性和工作流分析
Kristijan Skok1, Lukas Scheipner2, Sebastian Mannweiler1
1Diagnostic and Research Institute of Pathology, Medical University of Graz, Graz, Austria.
BJU international
|February 8, 2026
概括
光共聚焦显微镜 (HS) 在机器人辅助激进前列腺切除术期间显示出高特异性用于手术内边缘评估. 阴性HS结果具有高度可靠性,可能减少冷截面的使用,并保存组织用于分子测试.
科学领域:
- 尿瘤学 尿瘤学
- 手术病理学的外科病理学
- 医学成像医学成像
背景情况:
- 在神经节约型机器人辅助激进前列腺切除术 (RARP) 期间,手术内边缘评估 (IME) 是至关重要的,以确保完全切除瘤.
- 传统的冷截面 (FS) 分析在分子测试的速度和组织保存方面存在局限性.
研究的目的:
- 为了比较光共聚焦显微镜 (Histolog®扫描仪[HS]) 的诊断准确性,工作流程可行性和下游兼容性,与RARP中的IME的冷截面 (FS) 相比.
- 评估HS作为FS的潜在替代品或辅助工具,用于进行业内利率评估.
主要方法:
- 一项前性研究涉及66名接受RARP治疗非转移性前列腺癌的患者.
- 使用HS和FS进行了手术内边缘评估.
- 对诊断性能进行了整体组织学评估,并对IHC和NGS的组织兼容性进行了评估.
主要成果:
- 对于确定的呼叫,HS表现出高特异性 (98.1%) 和负预测值 (96.4%).
- 敏感度为60.0%,其中10.6%的病例被归类为可疑并由FS解决.
- 在下游免疫组织化学 (IHC) 和下一代测序 (NGS) 分析中,HS处理没有损害组织完整性.
结论:
- 光共聚焦显微镜 (HS) 在RARP中为IME提供高特异性和NPV,允许对负边缘进行自信的解释.
- HS可能会减少对FS的需求,特别是对于负边缘,同时保留用于分子诊断的组织.
- HS显示了将其集成到多式联络瘤工作流程中的潜力,从而增强了手术内决策.
相关概念视频
Confocal Fluorescence Microscopy
21.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.2K
Immunofluorescence Microscopy
13.7K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
13.7K
Matrix-Assisted Laser Desorption Ionization (MALDI)
1.2K
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
1.2K
Two-Dimensional Microscopy in Microbiology
1.6K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.6K
Atomic Force Microscopy
4.5K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.5K
Overview of Microscopy Techniques
16.9K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
16.9K

