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在水牛COCs的体外成熟过程中,甲素B的表达:亡和发育能力

Asit Jain¹, Tripti Jain¹, Sachinandan De²

¹Nanaji Deshmukh Veterinary Science University, Jabalpur, 482001, Madhya Pradesh, India.

Journal of assisted reproduction and genetics

|February 8, 2026

概括

在成熟期间在水牛卵细胞复合体中抑制甲素B (cath B) 改善了胚胎发育. 补充E-64可降低细胞亡并提高胚胎细胞质量,有助于体外胚胎生产.

科学领域:

生殖生物学生殖生物学
动物生物技术动物生物技术
细胞和分子生物学细胞和分子生物学

背景情况:

积卵细胞复合体 (COCs) 对卵细胞成熟和胚胎发育至关重要.
累积细胞中的亡可能会对水牛胚胎潜力产生负面影响.
甲素B (cath B) 在细胞过程中发挥作用,包括细胞亡.

研究的目的:

为了研究甲素B (cath B) 抑制对水牛COCs中亡标记物的作用.
为了确定甲基B抑制是否提高了在体外成熟 (IVM) 期间水牛卵细胞的发育潜力.

主要方法:

牛COCs (等级A和B) 在体外培养与或没有E-64 (cath B抑制剂).
用qRT-PCR分析了乙基因和亡调节者的基因表达.
蛋白质水平和细胞亡通过免疫染和TUNEL测定进行了评估.
在IVM,体外受精 (IVF) 和体外培养 (IVC) 后评估了发育能力.

主要成果:

与A等级相比,B等级的COC显示出较高的CathB和亲细胞灭绝基因表达.
E-64显著降低了B细胞和亲细胞亡的基因表达,并降低了累积细胞中的细胞亡.
补充E-64改善了胚胎囊的产量和质量,特别是在A级的COC中.

结论:

用E-64抑制甲基B有效地调节IVM期间水牛积分细胞的亡.
这种方法提高了水牛胚胎的发育能力,为改善体外胚胎生产提供了洞察力.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.布法罗水牛在线观看卡瑟普辛B是B的类型之一.E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-64 E-65 E-65 E-65 E-64 E-65 E-64 E-65 E-65 E-65 E-65 E-65 E-66 E-65 E-65 E-66

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