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在Caco-2细胞培养中,益生菌介导的消化格里亚丁和33-mer的保护作用
Anjali Jaglan1, Gunjan Sadera1, Anju Nagpal2
1Department of Microbiology, Central University of Haryana, Mahendergarh, India.
Molecular nutrition & food research
|February 8, 2026
概括
某些益生菌,如 Bacillus subtilis AJG10 和 Bacillus tequilensis AJG23,可显著降低免疫性质. 这减少了炎症并改善了肠道细胞的活力,为治疗乳性疾病提供了潜在的潜力.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 质蛋白质的消化产生免疫性,触发在乳病 (CeD) 中的炎症反应.
- 肠道上皮细胞受到这些质引起的细胞毒性和炎症反应的影响.
- 患有CeD的易感个体对质酸有不良反应.
研究的目的:
- 为了研究特定益生菌菌株Bacillus subtilis AJG10和Bacillus tequilensis AJG23对素和33-mer的保护作用.
- 评估这些益生菌进一步降解质衍生免疫性的能力.
- 评估益生菌治疗对Caco-2肠道细胞反应的影响.
主要方法:
- 利用Caco-2细胞模型来模拟肠道环境.
- 使用SDS-PAGE和质谱学分析了降解.
- 评估了Caco-2细胞活力,氧化 (NO) 生产和促炎性细胞因子表达.
主要成果:
- 益生菌治疗显著降解了免疫原性片段.
- 观察到氧化 (NO) 生产量减少了44%-53%.
- 显示27% - 37%的炎症性细胞因子表达减少,细胞活性的整体改善.
结论:
- 细菌亚细菌AJG10和Bacillus tequilensis AJG23显示出在化有毒质的潜力.
- 益生菌的干预可以减轻消化的格里亚丁和免疫性的细胞毒性和炎症作用.
- 这些发现表明,益生菌在治疗乳病方面发挥着有前途的作用.
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