甲基帕拉本对积分细胞DNA完整性和猪卵细胞发育能力在体外的影响
Yenny Ramírez-Jara1, Brenda Casado2, Miguel Betancourt2
1Graduate Program in Biological and Health Sciences, Metropolitan Autonomous University, Mexico City, Mexico.
Journal of applied toxicology : JAT
|February 9, 2026
概括
甲基 (MePB),一种常见的防腐剂,损害了猪卵细胞和细胞的活力和DNA完整性. 暴露于MePB显著损害体外成熟,受精和胚胎发育,影响生殖健康.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 烯 (PBs) 是消费品中广泛使用的防腐剂.
- 甲基 (MePB) 是一种普遍存在的,由于人类的高暴露,引起了健康问题.
- 对MePB对生殖健康的潜在影响需要彻底调查.
研究的目的:
- 评估MePB在猪卵细胞和细胞上的体外影响.
- 评估MePB对卵细胞和胚胎发育能力的影响.
- 确定MePB暴露与生殖健康问题之间的关系.
主要方法:
- 猪卵细胞在体外暴露于不同的MePB度 (0,500,750,1000μM).
- 评估细胞活力,细胞亡,亡,DNA/染色质完整性.
- 评估卵细胞成熟,受精和胚胎发育率.
主要成果:
- 在1000μM MePB下降了卵细胞活力;在750μM下降了累积细胞活力.
- 在所有测试度 (500-1000μM) 的累积细胞中增加了DNA损伤.
- 1000微米时卵细胞染色质完整性受损;从750微米开始,胚胎成熟和发育减少.
结论:
- MePB诱导对卵细胞和累积细胞的显著损伤.
- 暴露于MePB会损害胚胎发育能力和生存能力.
- 调查结果强调了MePB对生殖健康和生育能力的潜在不良影响.
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