MdWRKY75与MdWOX11相互作用,以调节果在盐应激下的根生长
Xiya Zuo1, Xiaoyun Zhang1,2, Li Fan1
1College of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F University, Yangling, Shaanxi, 712100, China.
The Plant journal : for cell and molecular biology
|February 9, 2026
概括
果植物通过根部发育途径适应盐的压力. MdWRKY75基因抑制根生长,而MdWOX11通过调节MdSAUR15来促进根生长,这对盐耐受性至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 无生物压力生理学 生物压力生理学
背景情况:
- 根系的发展对于植物的生长和耐压力至关重要.
- 果根适应盐压力的分子机制尚不清楚.
研究的目的:
- 确定调节果根适应盐压力的关键基因和途径.
- 阐明MdWRKY75在盐应激反应和根部发育中的作用.
主要方法:
- 转基因果中的基因表达分析.
- 酵母单杂交和ChIP-qPCR测定以确认蛋白质-DNA相互作用.
- 双分子光补充 (BiFC) 测定蛋白质与蛋白质相互作用.
主要成果:
- MdWRKY75 负面调节偶然的根形成和盐耐受性.
- MdWRKY75直接抑制了MdSAUR15的表达方式.
- 在盐应力下,MdWOX11增强了根形成,并对抗MdWRKY75抑制MdSAUR15.的作用.
结论:
- 一个新的MdWOX11-MdWRKY75-MdSAUR15调节模块控制了果根适应盐压力的过程.
- 该模块提供了改善果品种盐耐受性的潜在目标.
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