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在牛奶中检测和量化细菌金属蛋白酶AprX的技术:一篇评论
Aritra Sinha1,2, Alan L Kelly1,2
1School of Food and Nutritional Sciences, University College Cork, Cork, Ireland.
Comprehensive reviews in food science and food safety
|February 9, 2026
概括
热稳定的金属蛋白酶AprX导致乳制品腐烂. 本综述详细介绍了牛奶中AprX的检测方法,以帮助乳制品的质量控制和缓解策略.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 热稳定的金属蛋白酶 AprX 来自心理变性 Pseudomonas spp. 显著降低乳制品质量,特别是UHT牛奶.
- 有效检测和量化AprX对于防止腐烂和确保乳制品安全和保质期至关重要.
研究的目的:
- 审查和综合牛奶中AprX检测和定量技术的演变和当前状态.
- 提供各种方法的比较分析,以指导乳制品行业应用的选择.
主要方法:
- 传统方法的审查:免疫学测试 (例如,ELISA),酶活性测量,和生殖图.
- 现代分子方法的审查:基于PCR的方法 (例如,qPCR),质谱和先进的生物传感器平台.
- 基于灵敏度,特异性,速度,成本,可用性和工业适用性的比较评估.
主要成果:
- 像间接ELISA (LOD 21.0 ng/mL),多重qPCR和生物传感器等高度敏感的技术可以检测低度的AprX.
- 新兴趋势包括多体经济学,生物传感器小型化和人工智能用于数据解释,有望增强检测能力.
结论:
- 提出了对现有和新型AprX检测工具的批判性评估.
- 这一审查有助于乳业科学家和利益相关者选择最佳策略,以检测,监测和减轻牛奶和乳制品中AprX的破坏.
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