[-加载氧化物纳米颗粒在谷氨酸-兴奋毒性玻璃眼模型中的视网膜保护作用]
Lemeng Feng1,2,3, Yisong Chen4, Chao Wang5,6,7
1Ophthalmology Center, Xiangya Hospital, Central South University, Changsha 410008. lmfeng1997@126.com.
概括
装有的氧化纳米颗粒 (MgO-Zn NPs) 对玻璃眼治疗有希望. 这些纳米粒子保护视网膜质细胞免受伤害,并改善小鼠的视觉功能,作为潜在的眼内药物载体.
科学领域:
- 纳米医学是一种纳米医学.
- 眼科医生 眼科 眼科
- 神经科学是一个神经科学.
背景情况:
- 玻璃眼的特征是逐渐失去视网膜质细胞 (RGC).
- 目前的RGC保护策略是有限的.
- 纳米材料为RGC保护提供了作为药物输送载体的潜力.
研究的目的:
- 研究含氧化纳米颗粒 (MgO-Zn NPs) 对谷氨酸诱导的RGC损伤的神经保护作用.
- 评估MgO-ZnNP在玻璃眼瘤中的体外和体外生物相容性和治疗潜力.
主要方法:
- 准备和表征的MgO-Zn NP.
- 在R28细胞中使用CCK-8试验进行了体外细胞毒性评估.
- 在小鼠中建立了体内激发性视网膜损伤模型 (NMDA注射).
- 评估RGC亡 (TUNEL),视网膜结构 (HE),视觉功能 (F-VEP) 和基因/蛋白质表达 (RNA测序,免疫光).
主要成果:
- MgO-Zn NPs在R28细胞中没有表现出细胞毒性,在小鼠中表现出良好的眼膜耐受性.
- MgO-Zn NPs显著降低了NMDA诱导的RGC亡,并部分恢复了视网膜层结构.
- 在MGO-Zn NP治疗后,F-VEP测量显示视觉传导功能的部分恢复.
- RNA测序显示MgO-ZnNP调节PI3K-Akt-mTOR信号通路,从而降低p-Akt和p-mTOR的表达.
结论:
- MgO-Zn NPs对抗RGC损伤具有显著的神经保护作用.
- 这些纳米颗粒显示出潜力,作为一种双重功能药物治疗青光眼,提供视网膜保护和药物输送能力.
相关概念视频
01:25Glaucoma: Overview
1.4K
Glaucoma is an eye condition characterized by increased intraocular pressure that damages the retina and optic nerve, leading to irreversible blindness if left untreated. The human eye has various components, including the cornea, iris, pupil, lens, and optic nerve. Aqueous humor is secreted by the epithelium of the ciliary body in the posterior chamber and flows through the trabecular meshwork and canal of Schlemm, maintaining normal intraocular pressure. The trabecular meshwork and the canal...
1.4K
03:14Oxidation Numbers
42.9K
In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.9K
01:27Open Angle Glaucoma: Treatment
1.0K
In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...
Drugs such as carbonic anhydrase inhibitors, α2- and...
1.0K
01:15Pyruvate Oxidation
169.2K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
169.2K
01:14Antiepileptic Drugs: Glutamate Antagonists
1.0K
Glutamate is a fundamental neurotransmitter in the central nervous system, playing a vital role in neuronal communication and various cognitive processes. Glutamate stands as the principal excitatory neurotransmitter in the brain. Its presence is crucial for the communication between neurons, underpinning essential processes such as synaptic transmission, neuronal excitability, and plasticity. These functions are vital for higher-order cognitive processes, including learning and memory. The...
1.0K
03:11Oxidation-Reduction Reactions
75.8K
Oxidation–Reduction Reactions
75.8K