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Updated: Feb 10, 2026

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Extraction of Extracellular Vesicles from Whole Tissue
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组织衍生的细胞外囊泡定义了细胞癌的诊断生物标志物
Xinrui Wu1, Jiale Zhou1, Chen Jiang1
1Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Journal of extracellular vesicles
|February 9, 2026
概括
组织衍生的细胞外囊泡 (Ti-EVs) 显示为区分细胞癌 (RCC) 亚型的生物标志物具有前途. 尿路EV在检测低级和高级透明细胞RCC (ccRCC) 中取得了高准确性.
科学领域:
- 在瘤学瘤学.
- 生物标志物 生物标志物
- 细胞外囊泡 细胞外囊泡
背景情况:
- 组织衍生的细胞外囊泡 (Ti-EVs) 与瘤进展有关.
- Ti-EVs用于区分细胞癌 (RCC) 亚型的诊断实用性尚未得到充分证实.
研究的目的:
- 在Ti-EV中识别新的分子标记物,用于RCC亚型的差异诊断.
- 评估尿液细胞外囊泡 (EVs) 作为清细胞RCC (ccRCC) 的非侵入性生物标志物的潜力.
主要方法:
- 分析结合的瘤和正常组织及其相应的Ti-EVs.
- 鉴定特定的RNA和蛋白质标记物,用于表皮性RCC (pRCC),染色性RCC (chRCC),低度ccRCC和高度ccRCC.
- 用外部队列验证尿路EVs用于ccRCC检测.
- 整合单细胞测序数据以确定已识别的标记物的细胞起源.
主要成果:
- 针对pRCC (NEAT1,MMP15),chRCC (DSG2,AC026888.1),低等级ccRCC (NDUFA4L2,SERPINA1,VEGFA,EGLN3,CPE,C6orf223) 和高等级ccRCC (NDUFA4L2,APOC1,TGFBI,LINC00887) 的特定标记面板被确定.
- 尿路EV显示出高诊断性能,AUC为0.922的低度ccRCC和0.874的高度ccRCC.
- 单细胞分析显示,SERPINA1,VEGFA,APOC1和TGFBI来自与瘤相关的巨细胞,而NDUFA4L2来自低级和高级ccRCC的癌细胞.
结论:
- Ti-EVs具有特定的分子特征,可以区分RCC亚型.
- 尿路EVs代表了一个有前途的非侵入性工具,用于早期检测和差异诊断ccRCC.
- 了解EV标记物的细胞起源,可以了解瘤微环境的相互作用.
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