肝脏组织在芯片上建模肝脏再生和异种移植排斥
Abdul Rahim Chethikkattuveli Salih1, Arne Peirsman1,2, Danial Khorsandi1
1Terasaki Institute for Biomedical Innovation, Woodland Hills, California, USA.
Advanced materials (Deerfield Beach, Fla.)
|February 9, 2026
概括
研究人员开发了一种肝组织oid-on-a-chip (LToC) 模型,模仿人类肝功能和排斥. 这种器官芯片技术为研究肝移植和个性化医学提供了一个新的平台.
科学领域:
- * 生物医学工程 * 生物医学工程
- *再生医学是一种再生医学.
- * 移植免疫学 移植免疫学
背景情况:
- *目前的体外模型对于肝移植研究缺乏生理相关性.
- *器官芯片技术为开发可临床翻译的体外模型提供了一个有前途的途径.
- *需要先进的模型来研究肝脏再生和异种移植排斥.
研究的目的:
- * 设计一个血管化的肝脏组织oid-on-a-chip (LToC) 模型,复制人类肝脏组织.
- *利用LToC模型研究肝脏再生和异种移植排斥.
- *为免疫抑制药物测试和个性化移植医学建立一个平台.
主要方法:
- * 设计了一个微流体装置,在纤维素基质中使用人类肝脏前体细胞和内皮细胞.
- * 维持49天的动态培养以促进自我组装成血管化肝脏组织oids.
- *评估了组织骨架构,细胞活力,血管完整性,肝功能和细胞组成.
主要成果:
- * LToC模型自组装成具有叶片状结构的可 perfusable 微血管网络.
- * 已证明高细胞活力 (>95%),稳定的血管完整性和活跃的肝功能.
- *成功模拟了全原性T细胞诱导的肝脏排斥,包括降低活力,内皮干扰和炎症反应.
结论:
- * LToC模型为研究肝脏再生和免疫介导损伤提供了一个生理相关的平台.
- * 这种器官芯片系统可用于调查肝脏异位移植排斥.
- * LToC模型具有药物查和推进个性化移植医学的潜力.
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