相关实验视频
Updated: Feb 10, 2026

Model of Ischemia and Reperfusion Injury in Rabbits
Published on: November 3, 2023
一个可见/NIR-II双通道光探头用于调查在肝脏缺血-再输液损伤期间NLRP3炎症体和铁的作用
Jing-Jing Chao1, Li-Yuan Chen1, Jing Chen1
1Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Hunan Provincial University Key Laboratory for Environmental and Ecological Health, College of Chemistry, Xiangtan University, Xiangtan 411105, P. R. China.
研究人员开发了一种新的光探针 (PX-P),用于在肝损伤期间可视化脂质滴变化. 准NLRP3炎症酶和铁亡途径,保护肝脏免受损伤.
科学领域:
- 生物医学工程 生物医学工程
- 分子生物学分子生物学
- 肝病学 肝病学是一种肝病学.
背景情况:
- 肝缺血-再输液 (I/R) 损伤会导致严重的肝损伤和肝功能衰竭.
- NLRP3炎症酶和铁亡是I / R损伤的关键参与者,但它们的相互作用尚不清楚.
- 脂滴 (LD) 代谢与氧化应激和铁亡有关,使得LD动态对于理解I/R损伤至关重要.
研究的目的:
- 调查NLRP3炎症和ferroptosis在肝脏I / R损伤中的协同作用.
- 开发一种实时成像工具,用于在I/R损伤期间监测LD动态.
- 通过针对这些途径来探索潜在的治疗策略.
主要方法:
- 设计和合成了一种双通道光探测器 (PX-P) 用于特定的LD准和成像.
- 利用PX-P监测I/R损伤期间肝细胞和小鼠的LD极性变化.
- 使用可见/NIR-II双通道成像来观察LD积累和动态.
主要成果:
- PX-P能够实时可视化LD极性变化和与细胞器官相互作用.
- 在炎症和铁亡期间观察到LDs的动态变化,在正常细胞和癌细胞之间有区别.
- 在缺血和早期再注血期间,LD积累增加,随着长时间的再注血而减少.
- 同时抑制NLRP3炎症酶和铁亡途径显著降低了肝细胞损伤.
结论:
- PX-P作为一个多功能成像平台,用于跨尺度跟踪LD动态.
- 这项研究提供了关于LD代谢,NLRP3炎症酶和肝脏I/R损伤中的铁亡之间的相互作用的见解.
- 针对NLRP3炎症酶和铁亡,为I/R损伤提供了一个有前途的治疗策略.
相关概念视频
Hepatic Drug Clearance: Role of Transporters
The Role of Ion Channels in Neuronal Computation
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential....
Ion Channels
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
Effect of Hepatic Disease on Pharmacokinetics: Dose Adjustments Due to Hepatic Impairment
Hepatic Portal System
At its core, the hepatic portal vein is the result of a confluence of the superior and inferior mesenteric veins along with the splenic vein. Each of these veins has a unique role. The superior mesenteric vein is...
Channel Rhodopsins
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...

