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激活FAPα的NIR-I光探头,改善了瘤成像的水溶性
Cheng Xie1, Jing-Jie Wu1, Yurong Feng1
1Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, P. R. China.
Analytical chemistry
|February 9, 2026
概括
研究人员开发了新的光探针Sulfo-OHDF和Sulfo-FQCy7,用于检测纤维细胞激活蛋白-α (FAPα). 这些探头提供了提高的灵敏度和水溶性,使得有效的体内成像用于疾病诊断和治疗.
科学领域:
- 生物医学工程 生物医学工程
- 化学生物学 化学生物学
- 分子成像学分子成像学
背景情况:
- 纤维细胞激活蛋白-α (FAPα) 是癌症和其他疾病的有前途的生物标志物.
- 目前用于体内FAPα检测的分析工具有限.
- 开发敏感和特定的探针对于基于FAPα的诊断和治疗至关重要.
研究的目的:
- 开发具有增强性能的新型FAPα激活光探针.
- 为了评估这些探针在体内FAPα成像中的有效性.
- 为FAPα相关的生理和病理过程提供一个强大的工具.
主要方法:
- 设计和合成两个新型的FAPα激活光探头,Sulfo-OHDF和Sulfo-FQCy7,基于半氨酸和氨酸光体.
- 引入硫酸组以提高水溶性.
- 对探头性能进行评估,包括信号与噪声比,灵敏度和生物相容性.
- 在细胞,瘤组织和瘤携带小鼠中的体内成像实验.
主要成果:
- 新型探头与控制探头相比,显示了显著更高的信号噪声比率和对FAPα的灵敏度.
- 硫-OHDF显示光增强113.2倍,检测极限为1.59 ng/mL.
- 在各种生物模型中成功检测过度表达的FAPα,包括瘤组织和小鼠.
- 硫-OHDF在近红外激发/发射方面表现优越,生物相容性得到改善.
结论:
- 硫-OHDF和硫-FQCy7是有效的FAPα激活光探针,具有更好的水溶性和灵敏度.
- 硫-OHDF是一种高度敏感和生物相容的探头,适用于体内FAPα成像.
- 这些新型探针代表了推动基于FAPα的诊断和治疗的强大工具,特别是在瘤学中.
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