用不同的采样方法对重症监护病人的肠道微生物群的差异分析:系统性审查和元分析
Songlin Qiu1,2, Binyang Zheng1,2, Juan Pan1,2
1Department of Clinical Laboratory, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai, China.
Frontiers in microbiology
|February 9, 2026
概括
重症监护室 (ICU) 的患者表现出肠道微生物群的改变,多样性减少. 直肠拭子是一种可行的便替代品,用于分析ICU患者的肠道微生物群,产生类似的多样性结果.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 关键护理医学 关键护理医学
背景情况:
- 在重症监护室 (ICU) 患者中,肠道微生物组的组成因生理压力,肠道功能变化和抗生素使用而显著改变.
- 样品类型的变化 (例如,便,直肠拭子) 复杂化了ICU环境中肠道微生物群的分析.
- 了解这些微生物转变对于开发有针对性的干预措施至关重要.
研究的目的:
- 进行元分析,调查ICU患者肠道微生物群的变化,与健康个体相比.
- 评估不同样本类型是否会影响ICU患者肠道微生物群的分析.
主要方法:
- 进行了对11项研究的系统审查和元分析,比较了ICU患者和健康队列 (HC).
- 用随机效应模型分析数据,以量化微生物差异,包括α和β多样性.
- 具体评估了不同采样方法 (便与直肠拭子) 对微生物群分析的影响.
主要成果:
- 与健康个体相比,ICU患者的肠道微生物多样性和丰富性明显较低.
- 观察到β多样性的显著转变,蛋白质菌和菌的相对丰度增加,Firmicutes的数量减少.
- 分析显示,在比较便样本和直肠拭子时,α多样性没有显著差异.
结论:
- 与健康个体相比,ICU患者的肠道微生物群经历了显著的早期改变.
- 识别这些早期的微生物变化可以为ICU患者的预防和治疗策略的开发提供信息.
- 直肠拭子为评估ICU环境中的肠道微生物群和代谢组提供了便采样的实用和可靠替代方案.
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