化物是内皮细胞增殖,迁移和分化的内源性刺激剂
Anna Kieronska-Rudek1, Maria Petrosino1, Karim Zuhra1
1Section of Pharmacology, Department of Oncology, Microbiology and Immunology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.
Experimental biology and medicine (Maywood, N.J.)
|February 9, 2026
概括
内皮细胞从甘氨酸中产生化物,作为一种益血管性介质. 这种化物刺激血管内皮生长因子 (VEGF),促进细胞增殖,迁移和管形成,挑战其细胞毒性声誉.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 血管新生研究研究
背景情况:
- 化物通常被视为细胞毒性.
- 最近的研究表明,哺乳动物细胞,包括内皮细胞,通过从甘氨酸的溶酶体路径产生内源的化物.
- 低度或内源产生的化物可能具有调节作用.
研究的目的:
- 研究人类脉内皮细胞 (HUVECs) 中的化物生产.
- 确定内源性产生的化物在内皮细胞功能和血管生成中的作用.
- 探索化物,血管内皮生长因子 (VEGF) 和内皮细胞生物能学之间的关系.
主要方法:
- 在HUVEC中定量化化物生产.
- 用甘氨酸,杏仁素和曼德洛尼特里尔来评估对内皮细胞的影响.
- 测量VEGF蛋白质水平的方法.
- 评估ATP的产生,扩散和迁移.
- 使用氧科巴胺和 lysosomal 酸化抑制剂的抑制研究.
主要成果:
- HUVECs产生可检测的化物水平,由甘氨酸增强.
- 甘氨酸,杏仁素和曼德洛尼特里尔刺激内皮细胞的增殖,迁移和管形成.
- 化物对内皮细胞中的VEGF蛋白进行上调,这表明一个积极的反循环.
- 由VEGF刺激的细胞过程被化物清除剂和 lysosomal 酸化抑制剂抑制.
结论:
- 低度内源性产生的化物在内皮细胞中起到益血管性介质作用.
- 化物通过刺激VEGF途径和支持细胞生物能学来促进血管生成.
- 这一发现使化物的作用从纯粹的细胞毒性转变为血管化的潜在调节者.
相关概念视频
01:19Cell Migration
6.7K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.7K
01:09Cell Migration
18.8K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.8K
02:36Cells Coordinate Growth and Proliferation
5.1K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.1K
02:23Abnormal Proliferation
5.2K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.2K
01:35Cancer Cell Migration through Invadopodia
3.3K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.3K
01:21Chemotaxis and Direction of Cell Migration
5.1K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
5.1K