开发一种基于HPLC-PDA的分析方法,用于量化植物油中二酸的含量
Geun-Hee Cho1, Geon Oh1, Xiaolu Fu1
1Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Republic of Korea.
Food chemistry: X
|February 9, 2026
概括
二酸 (TDPA) 显示出作为食用油中长期作用的抗氧化剂的潜力,有效抑制脂质氧化. 一种经过验证的HPLC-PDA方法适用于在食品安全评估中监测TDPA.
科学领域:
- 食品化学 食品化学
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
背景情况:
- 食用油中的脂质氧化导致质量降低.
- 有效的抗氧化剂对于保持油品质量至关重要.
- 二酸 (TDPA) 是一种热稳定的化合物,作为一种潜在的食品添加剂,具有很大的兴趣.
研究的目的:
- 开发和验证HPLC-PDA方法来量化植物油中的TDPA.
- 在加速氧化条件下评估TDPA的抗氧化功效.
- 评估TDPA对监管监督和食品安全的适用性.
主要方法:
- 开发和验证了使用光电极阵列检测 (HPLC-PDA) 的高性能液态染色学.
- 方法验证包括特异性,线性,灵敏性,准确性和精度评估.
- 在60°C下进行了12天的加速氧化试验,将TDPA与BHA和BHT进行比较.
主要成果:
- 该HPLC-PDA方法显示出优异的性能 (R2>0.999) 与低检测 (0.44μg/mL) 和量化极限 (1.33μg/mL).
- 在50个商业植物油样本中没有检测到TDPA.
- TDPA表现出持续的脂质氧化抑制,保持其度的70%以上,在长期有效性方面表现优于BHA和BHT.
结论:
- 在食用油中,TDPA具有作为长效抗氧化剂的潜力.
- 经过验证的HPLC-PDA方法可靠地用于植物油中的TDPA量化.
- 在食品添加剂指定和安全性评估方面,TDPA需要进一步考虑.
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