活性精子DNA碎片化指数 (SDF) 在男性不孕症中提供了比总SDF更好的诊断准确度:一个横截面研究
Tianqin Deng1,2, Wenwen Yu3, Jilong Yao1,2
1Reproductive Medicine Centre, Shenzhen Maternity & Child Healthcare Hospital, Women and Children's Medical Center, Southern Medical University, Shenzhen, China.
Translational andrology and urology
|February 9, 2026
概括
活性的精子DNA碎片化 (SDF) 提供了比总的SDF更准确的男性不孕症测量方法. 9.79%的可行的SDF切断率证明了评估精子DNA损伤的卓越诊断性能.
科学领域:
- 生殖医学 生殖医学
- 安德罗学与人类学
- 精子生物学 精子生物学
背景情况:
- 精子DNA碎片化 (SDF) 是男性不孕症的一个关键生物标志物.
- 传统的SDF包括不可活性的精子,限制了临床相关性.
- 可行的SDF可能会提高男性生育能力的诊断准确性.
研究的目的:
- 探索一个可行的SDF切断的潜力.
- 评估可行的SDF对男性生育能力的预测价值.
主要方法:
- 对227名接受生殖评估的男性进行横截面研究.
- 精液分析遵循世卫组织第五版指南.
- 总和可活性的SDF使用精子染色蛋白结构试验测量,用远红色染色测量可活性.
- 在ROC曲线分析中,确定了最佳可行的SDF切线.
主要成果:
- 平均总SDF为21.89%,平均可行的SDF为10.31%.
- 可行的SDF与运动率负相关,与总SDF正相关.
- 最佳可行的SDF切线被确定为9.79% (AUC=0.813,灵敏度=76%,特异性=66%).
- 在总和可行的SDF分组之间发现了显著的差异.
结论:
- 可行的SDF提供了功能性精子DNA损伤的更准确的评估.
- 它评估了膜完好无损,受精能力强的精子中的DNA碎片化.
- 可行的SDF切断值为9.79%,显示出优越的诊断性能超过总的SDF.
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