内源MEP通路的代谢工程,以增强大肠杆菌中利科的产生
Xian Xu1, Hongyu Xing1, Hui Zhi1
1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210046, Jiangsu Province, China.
Synthetic and systems biotechnology
|February 9, 2026
概括
在这项研究中,Escherichia coli被改造为产生柳科,通过优化MEP途径和引入Deinococcus wulumuqiensis R12.12的基因,达到33.88倍的标位增加.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 微生物生物技术 微生物生物技术
背景情况:
- 微生物细胞工厂是异构型利科合成的关键.
- 基因源和基因通路调节显著影响烯产量.
- 优化2C-甲基-d-erythritol 4-酸盐 (MEP) 途径对于异烯酸的生产至关重要.
研究的目的:
- 通过优化MEP途径来增强大肠杆菌中的烯生产.
- 为了确定最优的基因来源,关键的利科生物合成基因.
- 建立一个强大的微生物细胞工厂,用于复杂的异oprenoid代谢物合成.
主要方法:
- 从Deinococcus wulumuqiensis R12引入了关键的烯生物合成基因 (crtE,crtB,crtI) 进入大肠杆菌.
- 优化发酵条件,识别酸盐作为一个关键的增强剂.
- 对MEP通路组件 (dxs,dxr,idi,ispA) 的不同基因来源进行选和共同表达,以加强该通路.
主要成果:
- 酸盐通过调节MEP途径基因表达,显著增强了柳科的产生和细胞生长.
- 优化了来自大肠杆菌MG1655的dxs和来自D. wulumuqiensis R12的idi的共同表达.
- 达到了293.70 mg/L (112.49 mg/g DCW) 的最大烯标位,比初始菌株增加了33.88倍.
结论:
- 这项研究为异质性胡卜素合成提供了宝贵的遗传资源.
- 优化MEP路径和选择合适的基因来源对于高产量烯生产至关重要.
- 这项工作为合成复杂的异oprenoid代谢物使用微生物细胞工厂建立了一个框架.
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