在Pseudomonas putida中,通过组合代谢工程实现了高水平的奇异素生产
Yuxin Zhang1,2, Meiyan Wang1, Kaijie Dou1
1College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China.
Synthetic and systems biotechnology
|February 9, 2026
概括
研究人员通过合成生物学增强了prodigiosin (一种生物活性颜料) 的生产. 一种经过工程改造的Pseudomonas putida菌株取得了高产量,克服了用于制药应用的本土Serratia marcescens分离物的局限性.
科学领域:
- 合成生物学 合成生物学
- 微生物生物技术 微生物生物技术
- 生物化学工程 生物化学工程
背景情况:
- 产素是一种来自Serratia marcescens的三聚烯颜料,具有宝贵的抗微生物,抗癌和抗疟疾特性.
- 对于实际的制药和工业应用而言,本地生产的产量太低,这阻碍了其广泛使用.
- 基因组分析证实了在S. marcescens分离物中存在prodigiosin生物合成基因集群.
研究的目的:
- 开发一个强大的平台,用于高水平的生产和过度生产.
- 设计一种微生物底盘,用于高效的异质生产奇异菌素.
- 为了显著增加超越本地S. marcescens能力的prodigiosin标位.
主要方法:
- 为异质表达设计了一个神奇的生物合成基因集群.
- 使用Pseudomonas putida KT2440底盘菌株用于颜料生产.
- 采用促进器工程,全基因组转子子子突变发生,以及发酵介质优化.
主要成果:
- 在使用工程P. putida菌株的shake-flask培养中达到665mg/L的最大prodigiosin标位.
- 在小规模水箱生物反应器培养中,进一步提高了prodigiosin产量至1161 mg/L.
- 与本地S. marcescens分离物相比,显示出明显更高的生产水平.
结论:
- 一个强大的合成生物学平台使得高水平的奇异的过度生产成为可能.
- 工程P. putida作为一个高效的底盘,用于 prodigiosin生物合成.
- 开发的平台可以适应过度生产其他原始化合物.
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