食油调节猪的炎症和氧化反应:与肝脏基因表达的关联
Fernanda Nery Ciconello1, Christopher Keith Tuggle2, Simara Larissa Fanalli3
1Luiz de Queiroz College of Agriculture - Department of Animal Science - Av. Pádua Dias, 11, 13418-900, Piracicaba, SP, Brazil.
像大豆,焦油和鱼油这样的食油会影响猪的炎症和氧化应激. 这些油中的特定脂肪酸会影响细胞因子水平和基因表达,影响猪模型中的免疫反应.
科学领域:
- 动物科学动物科学
- 营养科学 营养科学
- 免疫学 免疫学 免疫学
背景情况:
- 猪作为代谢研究的有价值的动物模型.
- 食用油中的脂肪酸显著影响脂质代谢和炎症反应.
- 了解这些影响对于解释代谢研究至关重要.
研究的目的:
- 评估大豆油 (SO),甘油 (CO) 和鱼油 (FO) 饮食如何影响猪的细胞因子水平和氧化应激.
- 为了将食油的影响与肝脏基因表达模式相关联.
- 阐明特定脂肪酸特征在免疫调节和氧化平衡中的作用.
主要方法:
- 27只雄性猪在98天的时间里被养了3%的SO,CO或FO补充的饮食.
- 血清和肝细胞因子 (IL-10,IFN-γ,IL-1β,IL-6,IL-18,TNF-α) 使用ELISA进行测量.
- 氧化状态通过ORAC和低酸清理能力进行评估;肝脏基因表达用加权基因共表达分析分析.
主要成果:
- 这种CO饮食增加了肝脏的干扰素- (IFN-γ) 和血清中低酸清除能力.
- 这种SO饮食提高了血清中介质素-10 (IL-10) 水平.
- 基因联合表达分析将SO与IL-10和瘤亡因子-α (TNF-α) 联系起来,CO与IFN-γ增加和IL-6/IL-18减少联系起来,FO与TNF-α减少联系起来.
结论:
- 食油根据其脂肪酸成分差异调节猪的炎症和氧化反应.
- 特定的脂质谱影响了猪模型中的免疫调节和氧化平衡.
- 这些发现有助于我们更好地理解在使用猪的代谢研究中饮食脂肪的影响.
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