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突流体细胞计数在72小时内保持稳定,无论储存温度如何:对PJI诊断的影响
Balato Giovanni1, Balboni Fiamma2, Ascione Tiziana3
1Department of Public Health, School of Medicine, Federico II University of Naples, Italy.
EJIFCC
|February 9, 2026
概括
同胞液白血细胞 (WBC) 计数和多态核中性细胞百分比 (PMN%) 稳定长达72小时,无论储存温度 (室温或4°C). 这种稳定性使得临床工作流程更具灵活性,尤其是在实验室直接访问有限的地方.
科学领域:
- 整形外科 整形外科 整形外科
- 传染性疾病 传染性疾病
- 临床病理学 临床病理学
背景情况:
- 周围假肢关节感染 (PJI) 诊断依赖于突液分析.
- 在各种储存条件下,流体白细胞 (WBC) 计数和多态核中性粒细胞百分比 (PMN%) 的稳定性尚未得到充分确立.
- 许多医疗机构缺乏即时的现场实验室能力,以便及时分析样本.
研究的目的:
- 评估储存温度 (室温与4°C) 对突液WBC计数和PMN%稳定性的影响.
- 为了确定这些参数在吸入后长达72小时的稳定性.
- 为周围假肢关节感染 (PJI) 的诊断方案提供信息.
主要方法:
- 对106个突液样本进行前性分析,这些样本来自于因疑似PJI而接受修复关节塑造手术的患者.
- 随机分配样品以室温 (RT) 或4°C储存.
- 在基线和72小时后使用自动化血液学分析仪测量WBC计数和PMN%.
主要成果:
- 突流体WBC计数和PMN%在RT和4°C时长达72小时保持稳定.
- 在冷藏样本中观察到略高的平均白细胞计数,但这些差异在统计学上并不显著.
- 由于存储变化,ICM 2018对PJI的诊断值没有发生样本重新分类.
结论:
- 同胞液WBC和PMN%的稳定性高达72小时,不管储存温度如何.
- 这些发现支持增加临床工作流程的灵活性,特别是对于没有立即现场实验室设施的机构.
- 立即的突液分析可能不严格要求PJI诊断,挑战现有的假设.
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