Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H01:13

meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H

6.7K
All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for...
6.7K
Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

6.0K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
6.0K
Plotting of Topographic Maps01:29

Plotting of Topographic Maps

548
Topographic maps represent the Earth's surface features using contour lines, which connect points of equal elevation to create a two-dimensional representation of three-dimensional terrain. Creating a topographic map requires a systematic approach.Begin by plotting a scaled grid and marking intersections corresponding to the survey's elevation data points. Assign elevation values at these intersections to build the base map. Next, determine contour levels using a consistent contour interval,...
548
Coordinates and Map Projections01:29

Coordinates and Map Projections

628
Coordinates and map projections are essential tools in accurately representing the Earth's surface for various applications, ranging from navigation to spatial analysis. The latitude and longitude coordinate system is a universally recognized framework for defining locations. Latitude specifies the distance of a point north or south of the equator, measured in degrees from 0° at the equator to 90° at the poles. Longitude indicates a location's position east or west of the prime meridian,...
628
2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

5.5K
Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
5.5K
Functional Groups02:45

Functional Groups

89.1K
Functional groups are a group of atoms with characteristic properties, which when linked to the carbon skeleton of a molecule, alter the properties of that molecule. For example, the presence of certain functional groups on a molecule will make them hydrophilic, whereas others will make them hydrophobic. These functional groups are an indispensable part of organic chemistry and important components of biological molecules, such as carbohydrates, proteins, lipids, and nucleic acids. Each...
89.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The ITCC-P4 PDX Platform Enables Preclinical Testing of Pediatric Cancers.

Cancer research·2026
Same author

Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin.

Molecular cell·2026
Same author

An MR1-specific nanobody capable of blocking MR1T cell activation.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Synaptotagmin 1 and Synaptotagmin 7 promote MR1-mediated presentation of <i>Mycobacterium tuberculosis</i> antigens.

eLife·2026
Same author

Use of hydrogen deuterium exchange mass spectrometry in tandem with modern structural biology.

The Biochemical journal·2026
Same author

A critical signaling role for diacylglycerol in phagocytosis of <i>M. tuberculosis</i>.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Feb 10, 2026

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research
06:08

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

1.7K

用纳米体绘制SARS-CoV-2核体功能的地图

Jules B Reyes-Weinstein, Timothy A Bates, Aidan Anastas

    bioRxiv : the preprint server for biology
    |February 9, 2026
    PubMed
    概括

    阿尔帕卡衍生的纳米体向SARS-CoV-2核体 (N) 蛋白质.

    更多相关视频

    Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
    03:53

    Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

    Published on: November 10, 2023

    2.0K
    Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
    08:41

    Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

    Published on: November 5, 2021

    3.4K

    相关实验视频

    Last Updated: Feb 10, 2026

    Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research
    06:08

    Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

    Published on: September 8, 2023

    1.7K
    Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
    03:53

    Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

    Published on: November 10, 2023

    2.0K
    Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
    08:41

    Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

    Published on: November 5, 2021

    3.4K

    科学领域:

    • 病毒学 病毒学
    • 结构生物学 结构生物学
    • 免疫学 免疫学 免疫学

    背景情况:

    • SARS-CoV-2核体 (N) 蛋白对于病毒复制和免疫调节至关重要.
    • 由于N蛋白的复杂性,对N蛋白域 (NTD,CTD,LINK) 的特定功能的理解是有限的.
    • 在细胞内组织和免疫逃避中N蛋白的作用需要进一步研究.

    研究的目的:

    • 开发和描述针对SARS-CoV-2 N蛋白的域特定纳米体 (VHHs).
    • 使用这些VHHs,研究N蛋白单个域的功能作用.
    • 探索针对N蛋白的VHHs的治疗潜力.

    主要方法:

    • 产生十二个来自羊的纳米体 (VHHs),针对N-终端域 (NTD),C-终端域 (CTD) 和链接器 (LINK) 区域.
    • 使用ELISA和生物层干涉测量的结合亲和特性表征.
    • 通过-交换质谱法 (HDX-MS) 和结构建模来绘制皮层映射.
    • 在体外和细胞内测试以评估VHHs对病毒感染和N蛋白相分离的影响.

    主要成果:

    • 开发了针对SARS-CoV-2 N蛋白的所有三个域的VHHs.
    • 证明细胞内VHHs可以抑制SARS-CoV-2感染.
    • 表明VHHs在体外中破坏了N蛋白相分离.
    • 证实了针对单个域的VHHs独立地阻断相凝和病毒复制.

    结论:

    • 域特定的VHH是剖析SARS-CoV-2 N蛋白质生物学的有效工具.
    • 对于病毒复制来说,N蛋白的所有三个域 (NTD,CTD,LINK) 都在功能上很重要.
    • 这些VHHs显示出针对SARS-CoV-2和其他冠状病毒的治疗应用的巨大潜力.