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相关概念视频

Introduction to Test of Independence01:21

Introduction to Test of Independence

3.0K
In statistics, the term independence means that one can directly obtain the probability of any event involving both variables by multiplying their individual probabilities. Tests of independence are chi-square tests involving the use of a contingency table of observed (data) values.
The test statistic for a test of independence is similar to that of a goodness-of-fit test:
3.0K
Hypothesis Test for Test of Independence01:16

Hypothesis Test for Test of Independence

8.2K
The test of independence is a chi-square-based test used to determine whether two variables or factors are independent or dependent. This hypothesis test is used to examine the independence of the variables. One can construct two qualitative survey questions or experiments based on the variables in a contingency table. The goal is to see if the two variables are unrelated (independent) or related (dependent). The null and alternative hypotheses for this test are:
H0: The two variables (factors)...
8.2K
Law of Independent Assortment02:03

Law of Independent Assortment

62.8K
While Mendel’s Law of Segregation states that the two alleles for one gene are separated into different gametes, a different question of how different genes are inherited remains. For example, is the gene for tall plants inherited with the gene for green peas? Mendel asked this question by experimenting with a dihybrid cross; a cross in which both parents are homozygous for two distinct traits resulting in an F1 generation that are heterozygous for both traits.
62.8K
Independent and Dependent Sources01:18

Independent and Dependent Sources

2.6K
In electrical circuits, sources play a crucial role in providing power for the operation of the circuit. These sources can be broadly categorized into two types: independent and dependent.
Independent voltage or current sources supply a fixed amount of voltage or current, respectively, which is unaffected by other elements within the circuit. These are represented using specific symbols. Independent voltage sources are symbolized with polarities (+ and -), indicating the direction of the...
2.6K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

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While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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相关实验视频

Updated: Feb 10, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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通过SS18::SSX进行BAF复合体独立基因激活:

Afroditi Sotiriou, Jinxiu Li, Sanya Middha

    bioRxiv : the preprint server for biology
    |February 9, 2026
    PubMed
    概括
    此摘要是机器生成的。

    这项研究表明,突肉瘤中的致癌性SS18::SSX融合蛋白可以独立于BAF复合体驱动转录. 针对EP300等联合激活剂为融合驱动的癌症提供了新的治疗策略.

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    Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
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    Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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    科学领域:

    • 在瘤学瘤学.
    • 分子生物学分子生物学
    • 癌症遗传学 癌症遗传学

    背景情况:

    • 突肉瘤是由SS18::SSX融合蛋白驱动的,它改变了BAF复合体的功能.
    • 据认为,BAF复合体对于SS18::SSX驱动的基因激活至关重要.

    研究的目的:

    • 调查BAF活动对突肉瘤细胞存活率和SS18::SSX转录的要求.
    • 为了确定SS18::SSX介导的瘤转录的替代机制.

    主要方法:

    • 针对BAF子单元的有针对性的降解和遗传删除.
    • 对SS18::SSX目标基因表达的分析.
    • 使用域特定的SS18::SSX突变.
    • 药理上抑制EP300/CREBBP的作用.

    主要成果:

    • BAF复合体的丧失对瘤细胞活力和SS18::SSX向基因表达的影响很小.
    • SS18::SSX 独立于 BAF 激活转录,依赖其 C 终端 QPGY 丰富的域.
    • SS18::SSX与EP300相互作用以促进转录.
    • EP300/CREBBP抑制抑制了SS18::SSX驱动的转录和减少了细胞存活率.

    结论:

    • 在突肉瘤中SS18::SSX介导的转录激活中,BAF活性并不必不可少.
    • 突肉瘤依赖于联合激活剂招募 (例如,EP300) 来进行瘤转录,独立于BAF.
    • 向EP300/CREBBP代表了针对突肉瘤和其他融合驱动癌症的潜在治疗策略.