一个双载体重组的SARS-CoV-2 Omicron BA.5用于体外和体内研究
bioRxiv : the preprint server for biology
|February 9, 2026
概括
一种新的双报告器SARS-CoV-2 Omicron BA.5 (rBA.5 ZsG-Nluc) 允许研究人员实时跟踪病毒感染. 该工具有助于识别有效的抗病毒药物和对当前SARS-CoV-2变种的抗体进行中和.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 持续出现的SARS-CoV-2令人担忧的变种 (VoC) 挑战了控制努力.
- 最近的VoC对现有的疫苗和抗病毒药物表现出耐药性,需要新的策略.
- 现有的评估新预防和治疗VoC的方法是有限的.
研究的目的:
- 为了生成一个重组的Omicron BA.5 SARS-CoV-2 (rBA.5 ZsG-Nluc) 编码ZsGreen和纳米化酶记者.
- 验证rBA.5 ZsG-Nluc用于跟踪病毒感染和评估抗病毒/抗体疗效.
- 建立一个平台,同时评估针对祖先和最近的SARS-CoV-2菌株的治疗方法.
主要方法:
- 利用基于细菌人造染色体 (BAC) 的逆遗传方法来创建rBA.5 ZsG-Nluc.
- 在细胞培养和K18 hACE2小鼠中评估病毒复制,致病性和记者基因表达.
- 采用体内成像系统 (IVIS) 来监测病毒传播和报告活动.
主要成果:
- rBA.5 ZsG-Nluc在体外和体内表现出与野生型BA.5相似的复制和致病性.
- ZsGreen和纳米化酶表达允许有效跟踪肺部和全身的病毒感染.
- 使用rBA.5 ZsG-Nluc获得的EC50和NT50值与使用rBA.5 WT获得的EC50和NT50值相似.
结论:
- rBA.5 ZsG-Nluc bireporter系统是跟踪SARS-CoV-2感染的一个可行的工具.
- 该系统有助于识别针对当前VoC的有效预防和治疗方法.
- 该平台可以同时评估针对不同SARS-CoV-2菌株的治疗方法.
更多相关视频
相关概念视频
Recombinant DNA
103.4K
Overview
103.4K
5-Number Summary
5.7K
In a dataset, the 5-number summary includes the minimum data value, the data value of the first quartile, the median data value or data value of the second quartile, the data value of the third quartile, and the maximum data value. These 5 data values can be visualized as a box and whisker plot.
In a box plot, the minimum and maximum data values represent the lower and upper whiskers in the graph, and the median is designated as the center of the box in the chart. The first quartile and third...
In a box plot, the minimum and maximum data values represent the lower and upper whiskers in the graph, and the median is designated as the center of the box in the chart. The first quartile and third...
5.7K
Viral Recombination
25.2K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
25.2K
Overview of Transposition and Recombination
19.4K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
19.4K
Conservative Site-specific Recombination and Phase Variation
6.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.9K
Homologous Recombination
63.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
63.4K


