质子独立过渡金属进口的结构和功能基础由规范的细菌Nramp传送器
bioRxiv : the preprint server for biology
|February 9, 2026
概括
自然耐药性相关的巨细胞蛋白 (Nramps) 是金属载体. 像BfraNramp一样,Clade B Nramps是质子独立的单载子,这表明质子合在Nramp函数中晚些时候进化.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 进化生物学 进化生物学
背景情况:
- 自然抵抗相关的巨细胞蛋白 (Nramps) 是必不可少的双价过渡金属载体.
- Nramps通常将金属吸收与质子共同运输结合起来,但这种机制的进化起源尚不清楚.
研究的目的:
- 为了研究Nramp传送器中质子合的进化起源.
- 来自 *Bacteroides fragilis* (BfraNramp) 的基底 Nramp 的结构和功能.
主要方法:
- 遗传学重建以确定 B 类 Nramps. 的进化位置.
- 对BfraNramp.的结构分析 (无金属和金属结合状态)
- 功能性测试用于评估金属运输活动和质子依赖性.
主要成果:
- Clade B Nramps是最基本的组,保留金属结合点,但缺乏关键质子通路残留物.
- BfraNramp有效地运输Mn2+和Cd2+,而不依赖于质子联合运输,膜电位或pH.
- 结构数据显示,BfraNramp中有一个向内开放的形状和一个独特的金属协调部位.
结论:
- Clade B Nramps的功能是作为质子独立的过渡金属单导体.
- 在Nramps中的质子合可能是在金属结合点建立后演变的,代表了后来的适应.
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