与等离子体相关的毒性基因和Enterococcus faecalis中的高水平 gentamicin 耐药性来自持续的内牙感染
Alexandra Castillo-Guevara1, Adriana Rodriguez-Ciodaro1, Catalina Méndez-De la Espriella2
1Center for Dental Research, Faculty of Dentistry, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia.
Journal of conservative dentistry and endodontics
|February 9, 2026
概括
高水平的 gentamicin 耐药性 (HLGR) 和 Enterococcus faecalis 中的特定毒性基因与持续的内牙感染有关. 识别沉默的HLGR对于有效治疗和预防失败至关重要.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 牙周内科医院 牙周内科医院 牙周内科医院
背景情况:
- 菌 (Enterococcus faecalis) 是持续性内牙感染 (PEI) 的常见原因之一.
- 抗微生物药物耐药性,特别是高水平的 gentamicin 耐药性 (HLGR),使治疗复杂化.
- 在E. faecalis的病毒性因素有助于感染的持久性.
研究的目的:
- 为了研究等离子体介导的HLGR,包括沉默的表型和关键毒性基因 (asa,esp,cylA) 在PEI中的E. faecalis之间的关联.
- 探索抗性和毒性基因的潜在共同选择机制.
主要方法:
- 从PEI中分离的E. faecalis被使用MicroScan系统对抗菌素敏感性进行了分析.
- 通过协同效应测试和PCR针对aac6') -Ie-aph2") -Ia基因进行了HLGR和静音HLGR表型的评估.
- 使用常规PCR检测出病毒性基因 (asa,esp,cylA),并采用混合数据因子分析 (MDFA).
主要成果:
- 从12.5%的样本中分离出E. faecalis,其中18.8%的样本表现出HLGR,包括两个静音表型.
- asa+ esp-cylA+基因型与耐HLGR菌株有显著的关联 (OR=15.6,P<0.001).
- MDFA确定了一个等离子体集群,将具有相同毒性概况的耐药/无声菌株联系起来.
结论:
- HLGR和毒性基因的同时出现表明PEI中的E. faecalis中的等离子体共同选择.
- 识别沉默的HLGR对于准确诊断和PEI的成功治疗至关重要.
- 了解这些遗传联系可以改善治疗内牙感染的治疗策略.
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