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基于EGOFET的适应传感器的单芯片多目标检测
Elena Yu Poimanova1, Elena A Kretova1, Anna K Keshek1,2
1Enikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393, Moscow, Russian Federation.
这项研究引入了aptamer修改的电解质门场效应晶体管 (EGOFETs) 用于多目标检测. EGOFETs可以使用单个aptamer识别元素检测pH值,银离子和流感A病毒.
科学领域:
- 生物医学工程 生物医学工程
- 纳米科学是一个纳米科学.
- 分子生物学分子生物学
背景情况:
- 液体多传感器对于各种应用至关重要.
- 将多个传感器集成到单个芯片上是一个活跃的研究领域.
- 经过Aptamer修改的电解质接场效应晶体管 (EGOFET) 提供了敏感生物传感的潜力.
研究的目的:
- 开发基于EGOFET的传感器,用于单芯片多目标检测.
- 为了利用具有pH依赖的形状切换的体,来感知多个分析物.
- 证明使用单一识别元素来区分不同目标的可行性.
主要方法:
- 制造由阿普坦尔修饰的EGOFETs.
- 使用具有i-动机DNA结构,表现出pH取决于形状变化的aptamer.
- 研究EGOFET对pH值变化,银离子 (Ag+) 和A型流感病毒的反应.
主要成果:
- EGOFET表现出对胺体形状变化的敏感性.
- 传感器通过依赖H+的i-motif组件检测到6-8范围内的pH值变化.
- 该设备根据独特的EGOFET信号区分pH,Ag+离子和流感A病毒.
- 在唾液和血等生物液中成功应用得到证实.
结论:
- 通过使用具有单个识别元件的EGOFET实现了单芯片多目标检测的概念验证.
- 这项工作为管理基于EGOFET的生物传感器属性铺平了道路.
- 它为在EGOFET平台上开发先进的单芯片多传感器奠定了基础.
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