通过病毒蛋白氨酸酸酶的寄生性割,向宿主细胞周期检查点蛋白Rad9A
Hongshuai Gao1,2, Mujuan Guo1, Xin Yang1
1Zhejiang Engineering Research Center for Biological Control of Crop Pathogens and Insects, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Ministry of Agriculture and Rural Affairs Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, State Key Lab of Rice Biology and Breeding, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
寄生黄蜂使用病毒蛋白氨酸酸酶 (PTP) 诱导亡,导致宿主昆虫如Plutella xylotella的割. 这会破坏宿主繁殖,并有助于生物控制工作.
科学领域:
- 分子生物学分子生物学
- 昆虫病理学 昆虫病理学
- 进化生物学 进化生物学
背景情况:
- 寄生虫割是一种常见的策略,寄生虫利用宿主生殖系统.
- 导致寄生虫割的分子机制尚不清楚.
- 寄生虫黄蜂经常利用病毒来操纵宿主生理.
研究的目的:
- 为了阐明由Cotesia vestalis在Plutella xylotella中诱导的寄生虫割的分子机制.
- 为了确定负责破坏宿主丸发育的病毒因素.
- 调查这些因素在其他昆虫物种中的保留作用.
主要方法:
- 宿主丸中的亡测定.
- 病毒蛋白氨酸酸酶 (PTP) CvBV_22-9.的鉴定和表征
- 为了研究Rad9A功能,CRISPR-Cas9基因编辑.
- 在Drosophila melanogaster中的表达分析.
主要成果:
- 由Cotesia vestalis寄生诱导Plutella xylotella丸的亡,由病毒PTPCvBV_22-9进行介导.
- CvBV_22-9与宿主蛋白Rad9A相互作用,破坏丸的发育.
- 敲除Rad9A会导致胚胎死亡和严重的丸缺陷.
- 在Spodoptera frugiperda和Drosophila melanogaster中观察到类似的机制,表明功能得到保护.
结论:
- 寄生虫黄蜂使用化病毒PTP作为伪酸酶来诱导Rad9A介导的亡,从而导致宿主割.
- 这种病毒策略会破坏宿主丸的发育和精子生成.
- 这些发现提供了对宿主寄生虫相互作用和类害虫潜在生物控制策略的见解.
相关概念视频
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein Kinases and Phosphatases
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
Lytic Cycle of Bacteriophages
Retrovirus Life Cycles
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....


