通过集成的计算流体动力学和机器学习框架,在双螺旋中优化氧气转移和水力动力学剪切的多目标优化
Qingfeng Gu1, Shuoyan Ji1, Yongqiang Liu1
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China.
Bioresource technology
|February 9, 2026
概括
智能生物反应器设计框架提高了氧气传输98.4%,并增加了β-胡卜素产量30.9%. 这种计算流体动力学和机器学习方法优化了混合生物反应器的生物制造.
科学领域:
- 生物技术和生物加工
- 化学工程是化学工程的重要组成部分.
- 计算科学 计算科学
背景情况:
- 高效的生物制造依赖于优化的生物反应器设计.
- 平衡氧气转移和剪切应力对于细胞活力和生产力至关重要.
- 目前的生物反应器设计往往涉及耗时的经验方法.
研究的目的:
- 开发一个智能设计框架,以优化双螺旋动生物反应器.
- 通过计算和机器学习方法,共同优化氧化 (kLa) 和剪切性能.
- 为了提高切割敏感生物工艺的产量,如β-胡卜素生产.
主要方法:
- 计算流体动力学 (CFD) 的集成用于性能模拟.
- 机器学习 (ML) 的应用,特别是人工神经网络 (ANN),作为代用模型.
- 使用非主导排序基因算法II (NSGA-II) 进行多目标优化.
主要成果:
- 该ANN替代模型实现了高预测准确性 (R2>0.97).
- 一个优化的不对称螺旋设计显著提高了kLa的98.4% (至312.5h-1).
- 发酵试验显示,在优化设计的情况下,β-胡卜素产量增加了30.9% (至3193 mg/L).
结论:
- 拟议的智能框架可以实现高效和合理的生物反应器设计.
- 这种方法成功地平衡了氧化和剪切性能,以改善生物过程结果.
- 该研究为通过优化生物反应器工程加强切割敏感生物过程提供了一条新的途径.
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