使用D2O标记的单细胞拉曼光谱解读甲基中的酸盐应激反应
Min Li1, Xiuxia Meng2, Erqi Nie3
1College of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255000, China; Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Qingdao New Energy Shandong Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
Bioresource technology
|February 9, 2026
概括
高水平的酸盐抑制了Methanosarcina barkeri.中的甲生产. 重水标记的单细胞拉曼光谱显示了在乙酸盐压力下代谢重编程和细胞功能中断,为微生物适应提供了洞察力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 挥发性脂肪酸的积累是无氧消化过程中的一个挑战.
- Methanosarcina barkeri表现出高的酸盐应激耐受性,但在单细胞水平上,潜在的机制尚不清楚.
研究的目的:
- 通过单细胞拉曼光谱来研究Methanosarcina barkeri对不同酸盐度的生理和代谢反应.
- 了解M. barkeri.中乙酸盐应激耐力的单细胞机制.
主要方法:
- 使用重水标记单细胞拉曼光谱法 (D2O-SCRS) 来分析M. barkeri.
- 检查了不同酸盐度梯度 (50,100和200毫米) 的反应.
- 应用多变量分析,包括主要组成部分线性差异分析,以解释拉曼光谱数据.
主要成果:
- 高度的乙酸盐 (200毫米) 显著抑制了甲的产生和微生物的生长.
- D2O-SCRS显示,随着酸盐水平的增加,代谢活性 (C-D比率) 的度依赖性下降.
- 多变量分析确定了明显的代谢重编程和表型转变,特定的生物标志物表明核酸,蛋白质和脂质功能的破坏.
结论:
- 乙酸盐压力显著影响M. barkeri的新陈代谢和单细胞水平的细胞功能.
- 在无氧消化过程中,D2O-SCRS是阐明微生物适应策略的宝贵工具.
- 研究结果提供了对复杂生态系统中考古物应激反应机制的见解.
相关概念视频
01:20Raman Spectroscopy: Overview
1.8K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.8K
02:02Responses to Salt Stress
14.7K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.7K
02:45Responses to Heat and Cold Stress
14.9K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.9K
01:26Raman Spectroscopy Instrumentation: Overview
1.4K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.4K
01:21Stress Response System
921
The stress response system, also known as the fight-or-flight response, is the body's automatic physiological reaction to perceived threats. Hans Selye introduced the concept of General Adaptation Syndrome (GAS) to describe the predictable pattern of changes that occur in response to stress. GAS consists of three sequential stages: alarm, resistance, and exhaustion. This model helps explain how chronic stress can contribute to health problems.
Alarm stage
In the alarm stage, the body's...
Alarm stage
In the alarm stage, the body's...
921
01:20Psychological Responses to Stress
720
Psychological responses to stress encompass the various cognitive and emotional reactions individuals experience when faced with challenging or threatening situations, such as a job loss. Prolonged exposure to stressors can disturb emotional balance, increasing negative emotions (e.g., anxiety and sadness) and diminishing positive emotions (e.g., joy and satisfaction). These persistent emotional shifts are associated with an increased risk of both physical illness and mental health issues, such...
720