显著的生物活性表面涂层调节了银纳米粒子暴露细胞中的慢性毒性和恢复途径
Amanda N Abraham1,2, Shakil Ahmed Polash1,2, Vipul Bansal1,2
1Sir Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Lab, School of Science, RMIT University, Melbourne, Victoria 3000, Australia.
ACS applied bio materials
|February 9, 2026
概括
银纳米粒子 (AgNPs) 的表面功能化显著影响其毒性. 黄素等生物活性涂层降低了慢性毒性,这表明了改善人类健康应用中AgNP安全性的方法.
科学领域:
- 纳米技术纳米技术
- 材料科学 材料科学 材料科学
- 毒理学 毒理学 毒理学
背景情况:
- 银纳米粒子 (AgNPs) 越来越多地用于消费者和生物医学产品.
- 由于AgNP的广泛应用,了解AgNP对健康的长期影响至关重要.
- AgNP的表面功能化可能会改变它们的生物相互作用和毒性.
研究的目的:
- 在人类细胞上研究具有不同表面功能的AgNP (氨酸,黄素,EGCG) 的急性和慢性毒性.
- 评估AgNP对氧化应激,细胞粘附和细胞循环进展的影响.
- 评估细胞恢复和间歇暴露对AgNP处理细胞的影响.
主要方法:
- 制备具有一致尺寸但多种表面涂层的AgNP:铁素 (Tyr),黄素 (Cur) 和表甲基素-3-酸盐 (EGCG).
- 评估急性和慢性毒性,氧化应激标志物,细胞粘附和细胞循环.
- 对暴露后细胞恢复的评估和通过细胞通道重复暴露AgNP的影响.
主要成果:
- 氨酸功能化的AgNPs (Tyr-AgNPs) 呈现低急性毒性但高慢性毒性,细胞需要多个通道才能恢复.
- 黄素功能化的AgNP (Cur-AgNP) 显示出最低的慢性毒性.
- 黄素和EGCG中基基的增强表面覆盖减少了AgNP的毒性.
结论:
- 对AgNP的生物活性表面涂层对于确定其生物相容性和长期细胞效应至关重要.
- 使用黄素等分子的功能化可以减轻银纳米颗粒的慢性毒性.
- 需要进一步研究表面修饰策略,以提高生物医学应用中AgNP的安全性.
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