固剂的选择会影响细胞块中死结体1的编程表达,这些细胞块来自患有转移性肺腺癌的多发性液
Mohammed S I Mansour1,2,3,4, Louise Pettersson1,2,5, Rita Pedersen1
1Department of Pathology and Cytology, Halland Hospital Halmstad, Halmstad, Sweden.
Cancer cytopathology
|February 9, 2026
概括
固定剂在细胞块中显著影响被编程死亡配体1 (PD-L1) 免疫细胞化学分析. 基于酒精的固定剂可能低估PD-L1阳性,可能导致癌症诊断中的假阴性结果.
科学领域:
- 细胞病理学 细胞病理学
- 在瘤学瘤学.
- 免疫组织化学 免疫组织化学
背景情况:
- 细胞块 (CBs) 的编程死亡配体1 (PD-L1) 免疫细胞化学 (ICC) 分析是一个不断增长的临床工具.
- 细胞学准备方法和固定剂各不相同,与标准化组织免疫组织化学不同.
- 这项研究检查了从恶性多流液 (MPE) 转移的肺腺癌 (AC) 细胞块中对PD-L1免疫活性的固定作用.
研究的目的:
- 研究不同固定剂对细胞块中PD-L1表达的影响.
- 用各种抗体和切断值来评估PD-L1免疫活性.
- 为了确定PD-L1分析在不同固定方法中的一致性.
主要方法:
- 从肺部AC患者中包括33个MPE的前景.
- 使用甲素,PreservCyt,CytoLyt和CytoRich Red固定剂制备四个匹配的CB.
- 用三种PD-L1抗体 (28-8,22C3,SP263) 进行免疫染色,并评估瘤比例得分和染色强度.
主要成果:
- 根据固定剂和抗体,一致性有所不同;PreservCyt和CytoLyt在≥1%的切断时显示出与甲素的中等一致性.
- 对于所有抗体,CytoRich Red 显示出实质性至非常好的一致性.
- 与甲素相比,基于酒精的固定剂导致PD-L1染色强度明显较低 (p < .001-.007).
结论:
- 在CB中PD-L1的ICC表达取决于所使用的固定剂和抗体.
- 基于酒精的固定剂可能导致低估PD-L1阳性,冒着假阴性结果的风险.
- 优化ICC协议对于可靠的诊断使用至关重要.
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