基于RPLC和HILIC的血液微样本的非向代谢学工作流
Pauline Couacault1, Michael Witting2,3
1Metabolomics and Proteomics Core, Helmholtz Zentrum München, Ingostädter Landstraße 1, 85764, Neuherberg, Germany.
Metabolomics : Official journal of the Metabolomic Society
|February 9, 2026
概括
血液微采样 (BμS) 为代谢学提供了一个不那么侵入性的替代方案. 优化的提取和LC-MS方法增强了从干血斑点和体积吸收微采样中检测各种代谢物的能力,改善了各种代谢途径的覆盖范围.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 代谢学 代谢学 代谢学
背景情况:
- 血液微抽样 (BμS) 是一种微侵袭的技术,用于分析内源代谢物.
- 干血斑点 (DBS) 和体积吸收微采样 (VAMS) 是BμS的关键格式.
- BμS适用于代谢学,为传统的血液和血采样提供了替代方案.
研究的目的:
- 开发和优化BμS的提取协议,用于非目标代谢.
- 建立强大的反相液态染色学/质谱学 (RPLC-MS) 和水友相互作用染色学/质谱学 (HILIC-MS) 方法.
- 为了提高对中极和非极极和极极化合物的代谢物覆盖率.
主要方法:
- 开发和优化了两种新的HILIC-MS/MS方法用于极性代谢物.
- 使用内部RPLC-MS方法对中极到非极地代谢物进行检测.
- 调查了五种提取程序,并优化了DBS和VAMS的工作流.
主要成果:
- 一种15分钟的HILIC-MS方法与柱子再平衡成功开发.
- 一个20%的H2O/80%的MeOH (v/v) 混合物与样品再水被证明是有效的代谢物提取.
- 该方法成功地通过各种途径检测了代谢物,包括氨基酸,酸和胆酸.
结论:
- 开发的提取和LC-MS方法提供了从BμS.中提供全面的代谢物概况.
- 这种方法提高了代谢物覆盖率,适用于DBS和VAMS.
- 优化的协议可使用微创血样进行非向的代谢学分析.
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