基因表达分析揭示了明显的PHB脱聚合机制和PHB循环在Rhodospirillum rubrum生长在乙酸盐和果糖上的更广泛参与
Hugo Fleuriot-Blitman1, Katerina Sabatova2, Véronique Amstutz1
1Institute of Life Sciences, University of Applied Sciences and Arts Western Switzerland Valais-Wallis (HES-SO Valais-Wallis), Sion, Switzerland.
在Rhodospirillum rubrum中,聚3-基酸盐 (PHB) 循环基因的表达不管PHB积累如何. PHB可能在能量储存之外发挥作用,影响基质同化.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- Rhodospirillum rubrum表现出一个动态的聚3-基酸盐 (PHB) 循环,积累和降解PHB.
- 在R. rubrum.rum中,在酸盐生长过程中观察到PHB积累,但在果糖生长过程中没有观察到果糖的积累.
研究的目的:
- 研究PHB周期基因表达与PHB积累/降解阶段之间的相关性.
- 为了比较乙酸盐与果糖的基因表达以及PHB缺乏突变的突变体.
主要方法:
- 野生型和突变型R. rubrum菌株的转录基因分析.
- 在不同碳来源 (乙酸和果糖) 上种植.
主要成果:
- 无论PHB含量如何,PHB循环基因都在酸盐和果糖上表达.
- 像phaZ1,phaZ2和 apdA这样的特定基因显示基质特定的表达模式.
- 在PHB缺乏突变体中,PHB循环基因仍然表达,影响挥发性脂肪酸同化通路.
结论:
- 在R. rubrum中PHB循环比以前想象的要复杂得多.
- PHB可能参与能量储存之外的生理过程,例如基质同化.
- 建议PhaR等PHB颗粒相关蛋白的调节作用.
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