作为治疗性siRNA传递系统,Carbosilane Dendrimers的血相容性可以跨越血脑屏障
Serafin Zawadzki1,2, Simon Suty3, Elżbieta Okła1
1Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz 90-236, Poland.
这项研究评估了一种用于阿尔茨海默病治疗的新型纳米载体的血红相容性. PEGylated carbosilane dendrimer及其 dendriplex对血液成分的不良影响最小,支持它们的临床潜力.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 药理学 药理学是指药理学的学科.
背景情况:
- 纳米载体对中枢神经系统 (CNS) 药物输送有希望.
- 临床翻译需要理解纳米载体与血液成分的相互作用.
- 血液相容性对于纳米载体的安全性和有效性至关重要.
研究的目的:
- 评估一种新型PEGylated carbosilane dendrimer (G3Si PEG6000) 和其树突的血液相容性.
- 评估纳米系统对血液风湿学,免疫反应和凝血的影响.
- 确定潜在的阿尔茨海默病治疗药物的安全性.
主要方法:
- 使用先进的分析技术进行全面的血红相容性测试.
- 评估细胞反应,细胞因子表达和血液学特性.
- 在生理环境中评估血液学参数和凝血动态.
主要成果:
- 在PBS中,dendriplex显示出明显较低的血液溶解比 dendrimers.
- 这两种配方在血中都是非溶血的;全血粘度适度增加.
- 纳米系统改变了凝血 (aPTT),但仍低于临床显著的值.
- 没有观察到对红细胞,血小板或白细胞的不良影响.
结论:
- 基化碳酸树脂和其树脂复合体表现出良好的血红相容性.
- 该纳米系统展示了针对中枢神经系统的治疗方法的翻译潜力.
- 治疗载荷和生物分子冠状形成影响纳米载体血液相互作用.
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