下一代流和下一代测序的互补作用,用于多发性髓瘤中可测量的残留疾病评估
Min-Seung Park1, Dae Jin Lim2,3, Hee-Jin Kim2
1Department of Laboratory Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
Journal of Korean medical science
|February 10, 2026
概括
下一代流量 (NGF) -MRD和下一代测序 (NGS) -MRD测定对于多发性骨髓瘤 (MM) 监测至关重要. 结合的MRD阴性预测了更好的无进展生存率,强调了这两种测定的重要性.
科学领域:
- 血液学 血液学 血液学
- 在瘤学瘤学.
- 分子诊断学 分子诊断
背景情况:
- 可测量的残留疾病 (MRD) 测定对于多发性骨髓瘤 (MM) 的预后和治疗指导至关重要.
- 将下一代流量 (NGF) -MRD和下一代测序 (NGS) -MRD试验进行比较对于优化患者管理至关重要.
研究的目的:
- 在MM患者中比较NGF-MRD和NGS-MRD试验的诊断性能和临床实用性.
- 通过NGF和NGS方法评估MRD检测的预后价值.
主要方法:
- 评估了来自41名MM患者的55个骨髓样本,以检测MRD.
- 在新鲜样本上进行NGF-MRD;使用LymphoTrack® IGH FR1测定对存档DNA进行NGS-MRD.
主要成果:
- 在NGF-MRD中,MRD检测率为43.6%,在NGS-MRD中为29.1%,一致性为67.3%.
- 两种测定组合的MRD阴性与MRD阳性 (40.0%,P=0.035) 相比,与明显更好的3年无进展生存率 (75.9%) 相关.
- 修订的国际分期系统第三阶段和联合MRD阳性与PFS有很强的关联.
结论:
- 在监测MM方面,NGF-MRD和NGS-MRD都发挥着关键的,互补的作用.
- 通过这两种方法分离的MRD阳性可以表明疾病进展,强调这两种测试的价值.
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