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波维-抗菌活性在体外对抗牙周细菌病原体
Thomas E Rams1, Chander S Gupta1
1Department of Periodontology and Oral Implantology, Temple University School of Dentistry, Philadelphia, USA.
波维- (PV-I) 在60秒内有效地减少了严重牙周炎生物膜中的有害细菌. 10%和5%的PV-I都抑制了病原体,同时保留了有益的菌株,支持其在牙周治疗中的使用.
科学领域:
- 微生物学 微生物学
- 牙周病学 牙周病学
- 抗菌剂 抗菌剂 抗菌剂
背景情况:
- 波维- (PV-I) 在体外表现出对牙周病原体的活性.
- 以前的研究经常使用更长的接触时间和参考菌株.
- 这项研究调查了PV-I在严重牙周炎的临床隔离物上的疗效.
研究的目的:
- 评估10%和5%PV-I对严重牙周炎的下生物膜细菌的抗菌作用.
- 用临床相关的60秒接触时间来确定疗效.
- 评估PV-I对总活力计数和特定牙周病原体的影响.
主要方法:
- 来自22名严重牙周炎患者的下生物膜样本被用10%的PV-I,5%的PV-I或没有PV-I治疗60秒.
- 剩余的PV-I被用硫酸二硫酸盐中和.
- 在无氧化后量化了细菌数量和特定的红色/色复合病原体;用MALDI-TOF MS识别了分离物.
主要成果:
- 与对照组 (14.8%) 相比,10%和5%的PV-I显著降低了总活力微生物数量 (60-68%) 和红色/色复杂病原体 (0.5%-0.7%).
- 在10%和5%的PV-I度之间没有发现显著差异.
- 红色/色复杂病原体在86.4%-95.5%的样本中被消灭;菌株菌株仍然普遍存在.
结论:
- 短期 (60秒) 的体外治疗10%或5%的PV-I有效地抑制了严重牙周炎的病原性下细菌.
- PV-I对抗抗生素耐药性和敏感性牙周病原体表现出抗菌活性.
- 这些发现支持PV-I作为牙周治疗的辅助剂,以促进更健康的口腔微生物群.
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