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一种高热稳定的古老的GH78Rhamnosidase有效地将黄类糖化物水解为果汁借款

Ali Shaikh-Ibrahim¹, Federica De Lise¹, Nicola Curci¹

¹Institute of Biosciences and BioResources, National Research Council of Italy, via P. Castellino, 111, Naples 80131, Italy.

Journal of agricultural and food chemistry

|February 10, 2026

概括

我们从温泉中确定了第一个耐热的古老α-L-rhamnosidase (ArRha). 这种酶有效地解生物活性黄类糖化物中的拉姆诺西尔连接,显示出工业应用的巨大潜力.

科学领域:

生物化学生物化学
酶学是一种酶学.
微生物学微生物学

背景情况:

α-L-Rhamnosidases是糖酸酸 (GHs),对于裂解终端α-L-Rhamnose残留物至关重要.
迄今为止还没有描述过任何古老的α-L-rhamnosidases,这限制了人们对它们在极端环境中的作用的理解.

研究的目的:

为了识别和描述第一个热稳定的古老α-L-rhamnosidase (ArRha).
为潜在的工业应用探索ArRha的酶活性和稳定性.

主要方法:

分析元基因组数据以识别Pisciarelli solfatara温泉中的新型酶.
纯化的古老α-L-rhamnosidase (ArRha) 的生物化学表征.
酶分析测试以确定基质特异性,最佳条件和稳定性.

主要成果:

对ArRha的鉴定和描述,ArRha是一种新型的耐热的古老酶,属于糖酸酸酶家族GH78.8.
ArRha有效地将α-1,2和α-1,6rhamnosyl链接化为生物活性黄类糖化物.
该酶表现出显著的热稳定性,广泛的pH活性,有机溶剂耐受性和不依赖金属离子.

结论:

这项研究报告了第一个超热稳定的古老GH78α-L-rhamnosidase (ArRha).
ArRha显示出工业应用的巨大潜力,包括酶果汁借记和可持续的黄类生物转化.
这些发现扩大了已知的α-L-rhamnosidases的多样性及其在极端环境中的能力.

关键词:

考古学是指古物质 (archaea) 是指古物质.碳水化合物活性酶酶.汁是一种汁.黄类化合物黄类化合物工业生物催化剂的工业生物催化剂在故事中,她讲述了她的故事.热稳定的酶.α-L-rhamnosidase 的使用情况.

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