挖掘Lentinula edodes干衍生的,双重抑制DPP-IV和ACE活动
Qiulan Zheng1, Qian Kang1, Haosu Zhang1
1School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
Bioscience, biotechnology, and biochemistry
|February 10, 2026
概括
来自Lentinula edodes茎的新型体显示出二次抑制双基酶-4 (DPP-IV) 和血管酶-1转化酶 (ACE). 这些安全的天然化合物有助于治疗2型糖尿病和高血压.
科学领域:
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
- 药理学 药理学是指药理学的学科.
背景情况:
- 2型糖尿病和高血压经常同时存在,需要针对这两种疾病的治疗.
- 抑制双基酶-4 (DPP-IV) 和血管素-1转化酶 (ACE) 是关键的治疗策略.
- 由于其氨基酸形状,Lentinula edodes干蛋白是生物活性的潜在来源.
研究的目的:
- 为了从Lentinula中提取和表征,Edodes具有双重DPP-IV和ACE抑制活性的干蛋白.
- 评估这些新型的疗效和安全性.
主要方法:
- 使用Bacillus subtilis蛋白酶进行Lentinula edodes干蛋白的酶性水解.
- 活性的分解和鉴定.
- 在体外测定DPP-IV和ACE抑制.
- 使用Caco-2细胞进行细胞毒性评估.
- 确定的固体相合成.
主要成果:
- 一个特定的分量 (F1-2) 显示出对DPP-IV (IC50 = 0.0910 mg/mL) 和ACE (IC50 = 0.0189 mg/mL) 的强烈抑制.
- 合成了三种新型 (EW-1,WR-2,LP-3),在1mg/ml的剂量下显示了两种酶的60%以上抑制.
- 合成的体对Caco-2细胞没有显著的毒性.
结论:
- 从Lentinula edodes茎中提取的具有显著的双重DPP-IV和ACE抑制特性.
- 这些是无毒的,是功能性食品或药品的有希望的候选者.
- 这项研究支持开发用于管理2型糖尿病和高血压的天然健康产品.
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