从使用智能手机基于纸片上的流量观测的人类血快速抗血测定
Ashley K Mathews1, Allison J Eby1, Jocelyn Reynolds2
1Department of Pharmacology, Perfusion Sciences Program, College of Medicine, The University of Arizona, Tucson, Arizona, United States of America.
PLOS digital health
|February 10, 2026
概括
一种基于智能手机的纸片新型检测方法可以快速检测人体血液中的抗血素 (AT) 水平. 这种便携式方法将毛细血管流速与AT度相关联,有助于管理抗凝状态.
科学领域:
- 生物医学工程 生物医学工程
- 临床化学 临床化学
- 临床诊断 临床诊断 临床诊断 临床诊断
背景情况:
- 抗血素 (AT) 对于调节血液凝固至关重要.
- 现有的AT检测方法缺乏可移植性和临床使用的速度.
研究的目的:
- 开发一种新的,快速的,便携式的方法来检测抗血素 (AT) 水平.
- 为了利用基于智能手机的毛细血管流观测在纸片上的AT量化.
主要方法:
- 开发了一种基于智能手机的测试,使用纸芯片进行AT检测.
- 相对应的毛细血管流速与AT度在的PBS和人体血中.
- 通过智能手机光显微镜测量粒子免疫聚合的验证结果.
- 通过使用人类血液样本,将新型测试与ELISA进行了比较.
主要成果:
- 在PBS (R2=0.988) 和血 (R2=0.973) 中,流速与AT度之间的高线性得到证明.
- 在等离子体试验中观察到钟形曲线,反映了抗体-抗原沉原理.
- 证实了毛细血管流速与粒子免疫聚合之间的正相关性.
- 对于人类血液样本,与ELISA显示出强烈的相关性,表明了测试有效性.
结论:
- 基于智能手机的毛细血管流量检测提供了一个近乎实时的,便携式的,简单的AT水平检测方法.
- 这种测试有可能解决确定AT缺乏和管理抗凝血的需要.
- 该技术需要最小的设备 (纸芯片,智能手机) 和一个单一的输管步骤.
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