针对加强登革热监测的分子检测算法的比较分析:一个现场案例评估
Vidal Felices1, Steev Loyola1,2, Crystyan Siles1
1U.S. Naval Medical Research Unit SOUTH, Lima, Peru.
The American journal of tropical medicine and hygiene
|February 10, 2026
概括
在BioFire®全球发烧面板 (GFP) 显示高精度检测登革热病毒 (DENV) 在现实世界的设置. 使用qRT-PCR和GFP的顺序测试策略提供了成本,时间和性能的最佳平衡.
科学领域:
- 临床诊断 临床诊断 临床诊断
- 传染病监测 传染病监测 传染病监测 传染病监测
- 分子生物学分子生物学
背景情况:
- 生物Fire®全球发烧面板 (GFP) 是用于检测病原体的多重测试,包括登革热病毒 (DENV).
- 现实世界GFP的绩效数据有限,特别是在特有地区.
- 准确及时诊断DENV对于患者管理和公共卫生至关重要.
研究的目的:
- 评估BioFire®全球发烧小组 (GFP) 对登革热病毒 (DENV) 检测的实际性能.
- 使用监测数据,将GFP与实时逆转录酶聚合酶连锁反应 (qRT-PCR) 进行比较.
- 评估不同的测试算法,将qRT-PCR和GFP结合起来,以获得最佳的诊断效用.
主要方法:
- 分析了来自秘鲁亚马逊地区224名急性发烧患者的登革热监测数据.
- 使用qRT-PCR (血清) 和GFP (全血) 检测到DENVRNA.
- 开发了两个参考标准:复合参考标准 (CRS) 和隐性类分析 (LCA),其中最好的LCA模型包括病毒载荷替代物.
主要成果:
- 使用qRT-PCR或LCA作为参考,GFP显示DENV检测94.5%的灵敏度和75.7%的特异性.
- 使用CRS作为参考,GFP显示95.6%的灵敏度和100%的特异性.
- 一种序列测试算法 (qRT-PCR,其次是负的GFP) 实现了99.3-100%的灵敏度和75.7-100%的特异性,提供了最佳的性能-成本-时间平衡.
结论:
- 在BioFire®全球发烧面板是一个可靠和高性能工具,用于在现实世界的临床环境中检测DENV.
- 结合qRT-PCR和GFP的顺序测试算法为DENV诊断提供了一个有效的策略.
- 尽管GFP具有诊断优势,但其成本可能是它在资源有限的地区广泛使用的障碍.
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