相关实验视频

Updated: Feb 12, 2026

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

马胎盘的单细胞地图揭示了细胞异质性和与妊娠阶段相关的程序

Margo H Verstraete¹, Jamie K Norris², Shavahn C Loux³

¹Department of Population Health and Reproduction, School of Veterinary Medicine, University of California Davis, Davis, CA, USA; Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

|February 10, 2026

概括

这项研究使用单细胞RNA测序绘制马胎盘细胞的地图,揭示了不同的细胞类型和从怀孕早期到晚期的发育变化. 它为了解马的怀孕和疾病提供了一个关键资源.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
哺乳动物比较生物学

背景情况:

胎盘对于影响发育和健康的母胎相互作用至关重要.
马 (Equus caballus) 胎盘的细胞组织尚未得到充分理解.
马类表皮形胎盘为胎盘研究提供了一个独特的模型.

研究的目的:

创建一个全面的单细胞转录基因马胎盘在整个妊娠期的图谱.
在马胎盘内识别和描述不同的胎儿细胞群.
调查胎盘细胞组成和基因表达的妊娠阶段相关变化.

主要方法:

单细胞RNA测序 (scRNA-seq) 在马类胆 ?? 状组织上进行.
从第一季度 (n=1) 和末期 (n=2) 胎盘中采集了样本.
生物信息分析被用来生成细胞地图,并确定细胞群.

主要成果:

确定了四种主要的胎儿细胞系: trofhoblast, stromal, endothelial 和 Hofbauer-like 细胞.
揭示了十种转录上不同的细胞种群,包括新的异质性.
在早期和终期胎盘之间观察到细胞组成和基因表达的显著差异,终期胎盘显示免疫和重塑通路的增加.

结论:

这项研究介绍了马胎盘的第一个高分辨率单细胞转录基因图谱.
它提供了对胎盘细胞多样性和马的发育动态的基本见解.
这本地图书作为一个基础,用于比较的胎盘生物学和马妊娠障碍研究.

关键词:

霍里奥尔朗托伊斯 (Chorioallantois) 是一个上位的胎盘上发生了什么?马类动物马类动物第一个三个月的胎盘.术语胎盘胎盘是什么意思我们的 scRNAseqq.

更多相关视频

Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic PolyI:C

Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic PolyI:C

Published on: March 25, 2016

Ex Vivo Perfusion of the Rodent Placenta

Ex Vivo Perfusion of the Rodent Placenta

Published on: May 30, 2019

相关概念视频

Diabetes Mellitus: Type 2 and Gestational

Diabetes Mellitus: Type 2 and Gestational

Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...

Stages of Infection

Stages of Infection

Stages of infection describe what happens to a susceptible host once a pathogen invades the human body. The stages of infection are incubation, prodromal, illness, stage of decline, and convalescence. The incubation stage is the period from exposure to a pathogen until symptoms start. The infected person is unaware of impending illness as the pathogens grow and multiply within the body. The duration may vary depending on the type of infection. The incubation period of measles averages ten to...

Stages of Sleep

Stages of Sleep

Sleep progresses through distinct stages, each characterized by specific brain wave patterns and physiological responses ranging from wakefulness to stages of non-rapid eye movement, known as non-REM, to rapid eye movement, referred to as REM. Understanding these stages helps in recognizing how sleep supports various bodily and cognitive functions.
Before sleep begins, in wakefulness, the brain exhibits primarily beta waves, which are high in frequency and low in amplitude, indicating alertness...

Stages of General Anesthesia

Stages of General Anesthesia

Various sedation levels offer significant advantages in facilitating procedural interventions for patients undergoing medical or invasive surgical procedures. These levels span from anxiolysis to general anesthesia, providing a spectrum of sedative effects to cater to specific patient needs. Anxiolysis reduces anxiety and is achieved through minimal sedation, enabling patients to remain awake and responsive while feeling more at ease during the procedure. This level can benefit minor...

Cellular Differentiation

Cellular Differentiation

How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...

Cellular Respiration

Cellular Respiration

Cellular respiration is a crucial metabolic process through which cells obtain energy from organic substances, mainly glucose, to produce adenosine triphosphate (ATP). This process includes the oxidation of substrates and the transfer of electrons to a separate electron acceptor, facilitating ATP synthesis through a sequence of biochemical reactions.Glycolysis: The Initial StepGlycolysis is the first stage of cellular respiration, occurring in the cytoplasm of both prokaryotic and eukaryotic...