青少年的昼夜基因表达:与同时发生昼夜干扰的关联以及心脏代谢风险指标的随后变化
Donghan Su1, Jaclyn M Goodrich2, Jennifer T Lee1
1Department of Nutritional Sciences, University of Michigan School of Public Health, Ann Arbor, MI, USA.
青少年的昼夜干扰对男性和女性的时钟基因表达和心脏代谢风险的影响不同. 这项研究揭示了睡眠模式,基因活性和代谢健康标志物之间的性别特异性联系.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 儿科内分泌学 儿科内分泌学
背景情况:
- 循环节障碍与代谢问题有关,特别是在青少年中,由于睡眠延迟和社交时差.
- 昼夜干扰对青少年心脏代谢风险的性别特异性影响尚不清楚.
- 有限的证据将青少年的昼夜干扰与特定的基因表达和心脏代谢结果联系起来.
研究的目的:
- 在青少年中调查昼夜干扰 (睡眠中点,社会时差) 和核心时钟基因表达之间的关联.
- 检查昼夜基因表达和心脏代谢风险因素的纵向变化之间的性别特异关系.
- 探索潜在的生物学机制,将昼夜干扰与青少年代谢健康联系起来.
主要方法:
- 203名青少年 (53%女性) 来自墨西哥城的ELEMENT队列.
- 七天的手腕动图用于睡眠评估;血液样本用于12个核心时钟基因的RNA测序.
- 在两年内,差异基因表达分析和线性回归将睡眠参数,基因表达和心脏代谢生物标志物 (脂肪,葡萄糖,脂质,血压) 联系起来.
主要成果:
- 较晚的睡眠中点与RORA,RORC,CLOCK和NR1D2.2的表达减少相关.
- 在两年内,观察到钟基因表达 (例如NR1D2,BMAL1,PER1-3) 和代谢生物标志物的变化之间存在性别特异关联.
- NR1D2显示了性别特异性联系:女性的禁食葡萄糖负相关性,男性的LDL和总胆固醇的积极相关性.
结论:
- 核心时钟基因表达与昼夜干扰和青少年心脏代谢风险因素的性别特定变化有关.
- 研究结果表明,有潜在的生物学途径将昼夜干扰与青少年心脏代谢健康联系起来.
- 强调在青少年昼夜研究中将性视为生物变量的重要性.
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