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一个特定于丸的双性基因参与了东方河,Macrobrachium nipponense的精子生成
Haifu Wan1, Lei Yu1, Shuohan Yang1
1College of Life Sciences, Hebei University, Baoding, China; Institute of Life Science and Green Development, Hebei University, Baoding, China; Hebei Innovation Center for Bioengineering and Biotechnology, Hebei University, Baoding, China.
International journal of biological macromolecules
|February 10, 2026
概括
研究人员在东方河Macrobrachium nipponense.se中发现了双性 (DSx) 基因. 这种基因对雄性的丸发育和精子生产至关重要,为水产养殖的进步提供了目标.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 甲动物生物学 甲动物生物学
- 分子遗传学 分子遗传学
背景情况:
- 东方河 (Macrobrachium nipponense) 呈现性二态性,雄性生长速度更快,使单性培养成为水产养殖的理想选择.
- 双性 (Dsx) 基因是性别决定和分化的关键调节者,在物种中保存,但它在十足类甲类动物中的作用尚不清楚.
研究的目的:
- 为了克隆和表征Dsx同类 (MnDsx) 在Macrobrachium nipponense.
- 研究MnDsx的表达,丸发育中的功能以及与其他分子的相互作用.
- 确定潜在的基因标,以控制性别和提高水产养殖产量.
主要方法:
- 对MnDsx基因进行克隆.
- 组织特异性表达分析 (包括眼切除的效果).
- 基因淘汰的RNA干扰 (RNAi).
- RNA测序 (RNA-seq) 和CUT&Tag测试用于识别目标基因.
- 拉向测定和LC-MS/MS用于识别蛋白质相互作用体.
主要成果:
- MnDsx mRNA主要表达在丸中,局部在生殖细胞中.
- 眼切除减少了MnDsx的表达.
- MnDsx knockdown显著降低了精子数量.
- 确定了1140个潜在的MnDsx点基因,这些基因参与丸发育和精子生成.
- 确定了63种MnDsx相互作用蛋白质,包括基因组.
结论:
- MnDsx在Macrobrachium nipponense的精子生成中发挥着至关重要的作用.
- MnDsx可以通过染色体重塑来调节基因转录.
- 这项研究提供了关于甲类的性别确定和水产养殖性别控制的潜在目标的见解.
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