快速和视觉检测Coxsackievirus A6使用反向转录多重交叉位移放大与纳米粒子生物传感器平台相结合
Yumei Cao1,2, Liang Qi2,3,4, Zhang Xu Jian2,5
1School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 561113, P.R. China.
一种新的测定方法,CVA6-RT-MCDA-LFB,可以快速准确地检测出Coxsackievirus A6 (CVA6),这是导致手足口病的主要原因. 这种有前途的护理点测试 (POCT) 为CVA6检测提供了解决方案,特别是在资源有限的环境中.
科学领域:
- 病毒学 病毒学
- 分子诊断学 分子诊断
- 生物技术是生物技术.
背景情况:
- 考克萨基病毒A6 (CVA6) 是幼儿手足口病 (HFMD) 的首要原因.
- 最近的CVA6爆发突显了迫切需要有效的诊断工具,因为目前没有疫苗或抗病毒治疗方法可用.
研究的目的:
- 开发和评估CVA6.6的新型快速检测试验.
- 与现有方法相比,评估试验的灵敏度,特异性和临床性能.
主要方法:
- 一种CVA6检测试验,CVA6-RT-MCDA-LFB,是通过将反转录多重交叉位移放大 (RT-MCDA) 与侧流生物传感器 (LFB) 结合而开发的.
- 该试验使用了十种针对CVA6 VP1基因的特定原始剂.
- 临床性能通过使用70个门拭子和42个喉拭子样本进行了验证.
主要成果:
- 在CVA6-RT-MCDA-LFB试验中,每个反应的分析灵敏度达到33个副本.
- 该试验表明100%的特异性,没有与其他病毒菌株的交叉反应.
- 临床评估显示与商业定量实时PCR (qRT-PCR) 百分之百一致.
结论:
- CVA6-RT-MCDA-LFB测定提供了一个快速 (不到1小时),简单和准确的CVA6检测方法.
- 它的高性能和速度使其成为一个合适的点检测 (POCT) 解决方案,特别是在资源有限的环境中.
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RACE - Rapid Amplification of cDNA Ends
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