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Updated: Feb 12, 2026

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

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Published on: August 25, 2018

通过使用16S rRNA测序来比较周植入炎患者的样本类型中的微生物组概况

Da-Mi Kim¹, Inpyo Hong¹, Joo-Yeon Lee¹

¹Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.

Journal of periodontal & implant science

|February 10, 2026

概括

围植入炎的微生物分析显示,炎症性结缔组织,牙裂液和牙下斑块具有相似的形状,与唾液不同. 唾液显示了较低的致病性细菌,特别是在深处的口袋里.

科学领域:

微生物学微生物学
牙周病学牙周病学
牙科植入物学牙科植入物学

背景情况:

牙周植入炎是牙科植入物的常见并发症.
已经进行了围部植入炎的微生物分析,但采样方法各不相同.
了解不同样本类型的周植入炎微生物群对于诊断和治疗至关重要.

研究的目的:

为了比较唾液中的微生物群落,牙裂液 (GCF),牙下斑块 (SGP) 和周围植入炎部位的炎症结合组织 (ICT).
为了确定特定于围植入炎病变的微生物特征.
评估不同样本类型中的微生物群落之间的相关性.

主要方法:

16S rRNA测序是在18名患有围植入炎的患者的唾液,GCF,SGP和ICT样本上进行的.
分析了微生物多样性 (α和β) 和分类概况.
评估了不同样本类型的微生物群落之间的相关性.

主要成果:

在ICT,GCF和SGP中的微生物群落相似,而唾液呈现出不同的特征.
唾液显示出较低比例的细菌类和较高的蛋白质细菌/活性细菌,特别是在深处的口袋.
在ICT和GCF/SGP之间发现了强烈的相关性,但不是与唾液.
关键的周植入炎病原体,如 Porphyromonas gingivalis 在 ICT 中比唾液更为丰富.

结论:

ICT,GCF和SGP在围植性炎中都存在类似的微生物群落.
唾液具有明显的微生物特征,与局部样本相比,植入周围病原体的丰度较低.
这些发现强调了样本类型在围植入炎微生物组研究中的重要性.

关键词:

16S rRNA 是一个16S rRNA.微生物组是一个微生物组.周植入体炎在周植入体炎.有关RNA测序的RNA测序

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