HLA-DPA1作为诊断生物标志物,可以区分早期和晚期出现的先兆子
Zhuna Wu1, Yajing Xie1, Weihong Chen1
1Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical University, No.34 ZhongShan North Road, Quanzhou, 362000, Fujian Province, China.
Scientific reports
|February 10, 2026
概括
机器学习确定了HLA-DPA1作为早期发病前症 (EOPE) 的关键免疫生物标志物. 在EOPE中HLA-DPA1的下调表明母胎免疫耐受性受损,提供了新的诊断和致病性见解.
科学领域:
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
- 计算生物学 计算生物学
背景情况:
- 孕前 (PE),特别是早期发病的PE (EOPE),与免疫系统调节失调密切相关.
- 识别可靠的免疫生物标志物对于理解EOPE病原和改善诊断至关重要.
研究的目的:
- 利用机器学习来发现EOPE的关键免疫生物标志物.
- 评估已识别的生物标志物的预测和诊断潜力.
主要方法:
- 利用了来自ImPort的mRNA表达数据 (GSE60438 + GSE75010) 和与免疫相关的基因.
- 进行了差异表达分析,基因本体学 (GO),KEGG丰富和蛋白质-蛋白质相互作用 (PPI) 网络分析.
- 应用LASSO和mSVM-RFE用于生物标志物选择,通过ROC曲线,名录和额外的数据集 (GSE22526,GSE74341,GSE190639) 进行验证.
- 使用CIBERSORT分析了免疫细胞透,并通过IHC和qRT-PCR验证了基因表达.
主要成果:
- 在晚发性PE (LOPE) 和EOPE之间鉴定出7个不同表达的免疫相关基因 (DIRG),包括HLA-DPA1.
- GO和KEGG的分析显示,T细胞增殖和免疫/代谢/心血管疾病中的丰富.
- HLA-DPA1被确定为EOPE的潜在诊断生物标志物,其AUC为0.758.
- 证实了EOPE胎盘组织中HLA-DPA1的下调,与特定的免疫细胞群相关.
结论:
- 免疫力在EOPE胎盘病原发生过程中起着至关重要的作用.
- HLA-DPA1是EOPE中与免疫细胞相关的关键免疫基因,为诊断和理解疾病机制提供了新的视角.
- 减少HLA-DPA1表达可能表明EOPE中的母胎免疫耐受性受损.
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