与动素相关的蛋白T3对于小鼠的宏体生物发生和精子功能是必需的
Andjela Kovacevic1, Eva Ordziniak1, Naila Umer1
1Institute of Pathology, Department of Developmental Pathology, Medical Faculty, University of Bonn, 53127 Bonn, Germany.
概括
在小鼠中,与动氨酸相关的蛋白T3 (ACTRT3) 缺乏会损害精子的发育,影响体形成和周核细胞结构. 这会影响男性的生育能力,因为它会破坏戈尔吉贩运和细胞骨组织.
科学领域:
- 生殖生物学 生殖生物学
- 精子发生是精子生成.
- 细胞生物学 细胞生物学
背景情况:
- 与动氨酸相关的蛋白T3 (ACTRT3) 是小鼠精子动物中周核 (PT) 的组成部分.
- 在男性生育能力和精子发育中,ACTRT3的确切功能在很大程度上是未知的.
研究的目的:
- 为了研究ACTRT3在男性生育能力和体生物生成中的作用.
- 阐明ACTRT3在精子生成中的功能背后的分子机制.
主要方法:
- 产生和分析Actrt3淘汰赛 (Actrt3-/-) 的小鼠.
- 免疫光显微镜,西方涂抹,共免疫沉和质谱学.
- HEK293T细胞转染以研究细胞骨效应.
主要成果:
- 在Actrt3-/-雄性小鼠中,表现出亚生育性,并存在形形成缺陷.
- 缺少ACTRT3会损害戈尔吉贩运和自流,由改变的标记物水平 (TGN46,GOPC,GM130,LC3B,CTSB,mTOR) 证明这一点.
- ACTRT3与PT蛋白 (ACTRT1,ACTRT2,ACTL7A,SPEM2) 和ZPBP相互作用,这表明它在PT结构和精子表面蛋白质定位中的作用. 细胞骨调节器 (CFL1,CNN1) 被丰富,而ACTRT3影响了F-actin的分布.
结论:
- 在精子生成过程中,ACTRT3对体生物发生,周核体结构和动蛋白重塑至关重要.
- 失去ACTRT3会导致Golgi流通和自流失,导致男性不孕.
- ACTRT3 作为 PT 内的蛋白质复合体的一部分,并影响细胞骨组织.
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