在玉米中长非编码RNA和microRNA之间的相互作用网络的计算预测,基于模糊的Tassel突变系的转录组
J Yan1, A Yu Pronozin2, D A Afonnikov3
1Novosibirsk State University, Novosibirsk, Russia.
Vavilovskii zhurnal genetiki i selektsii
|February 11, 2026
概括
这项研究揭示了非编码RNA (lncRNAs) 与玉米突变中的微RNA (miRNAs) 相互作用的时间. 这些相互作用作为竞争的内源RNA网络,为作物改进和耐压育种策略提供了新的见解.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 长非编码RNAs (lncRNAs) 调节基因表达,通常通过与微RNAs (miRNAs) 作为分子海绵进行相互作用.
- 这些相互作用的计算预测可以通过共同表达数据来改进.
- 由于Dicer-like1 (DCL1) 基因突变,玉米突变系的模糊 (fzt) 呈现出减少的miRNA表达.
研究的目的:
- 为了研究fzt突变系的玉米芽和皮组织中潜在的lncRNA-miRNA相互作用.
- 根据预测的相互作用构建竞争的内源RNA (ceRNA) 网络.
- 了解lncRNAs作为miRNA海绵在转录后调节中的作用.
主要方法:
- 分析了来自对照和第一种突变玉米组织的RNA测序 (RNA-seq) 数据.
- 转录组组合和差异表达分析确定了改变的lncRNAs.
- 机器学习 (PmliPred) 和RNA-RNA相互作用 (IntaRNA) 工具预测了miRNA-lncRNA相互作用.
主要成果:
- 在射击组织中发现了10种差异表达的lncRNA,其中9种在突变者中升级调节.
- 34个差异表达的lncRNA在毛组织中被确定,其中20个是上调调的.
- 预测的相互作用形成了ceRNA网络,显示 lncRNAs 结合多个miRNAs.
结论:
- 该研究阐明了玉米中的lncRNA-miRNA相互作用,有助于理解转录后调节.
- 已识别的lncRNAs作为海绵,调节miRNA活动.
- 这些发现为开发新的玉米育种策略提供了基础,以提高耐压力和生产力.
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