不同的储存条件和不同检测时间对通过流细胞计量对编程细胞死亡蛋白1的结果的影响
Clinical laboratory
|February 11, 2026
概括
储存条件和时间显著影响了在外周血液中检测细胞死亡蛋白1 (PD-1). 为了获得精确的流式细胞计 (FCM) 结果,在样品准备后,在4°C或4小时的室温下限制检测到8小时.
科学领域:
- 免疫学 免疫学 免疫学
- 临床诊断 临床诊断 临床诊断
- 生物医学分析 医学分析
背景情况:
- 准确检测编程细胞死亡蛋白1 (PD-1) 对于临床诊断至关重要.
- 流细胞计 (flow cytometry,FCM) 是一种常见的方法,用于对周围血液中的PD-1分析.
- 了解预分析变量的影响,如存储,对于可靠的结果至关重要.
研究的目的:
- 为了研究不同储存条件 (4°C与室温) 和检测时间对外围血液样本中PD-1表达的影响.
- 确定使用FCM进行PD-1分析的最佳存储和处理协议.
- 确保PD-1检测结果的准确性和稳定性.
主要方法:
- 68名住院患者的外周血液样本使用FCM进行了分析.
- 细胞悬浮液在4°C和室温下存储,保护免受光线的影响.
- 样品在准备后0,4,8和24小时进行分析,以评估PD-1在各种细胞群中的表达.
主要成果:
- 在4°C保存时,PD-1结果在长达8小时内没有显著变化,在24小时后显著下降.
- 在室温下储存4小时没有显著差异,但在8小时和24小时显著下降.
- 七个测量细胞群的稳定性,包括PD-1表达细胞,受储存时间和温度的影响.
结论:
- 检测PD-1的时间是样品后准备的关键.
- 对于存储在4°C的样品,检测应在8小时内进行.
- 对于存储在室温的样品,检测应在4小时内进行,以保持结果的准确性.
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