在合成和面包小麦胚胎质的修改的嵌套协会映射面板中剖析Fusarium头部虫耐药性
Anil Karmacharya1, Dhondup Lhamo2, Krishna Acharya3
1Department of Plant Sciences, University of California, Davis, CA, 95616, USA.
概括
研究人员在小麦中发现了Fusarium头部炎症 (FHB) 耐药性的新遗传位点. 这项研究利用了一种新的绘图面板,发现了三种潜在的新型耐药性局部和十六种已知的局部,增强了对这种破坏性疾病的小麦育种策略.
科学领域:
- 植物病理学 植物病理学
- 农业遗传学 农业遗传学
- 小麦育种 小麦育种
背景情况:
- 菌头部病 (FHB) 对全球小麦产量构成重大威胁.
- 由于FHB耐药性的狭窄遗传基础,现有的育种计划面临限制.
研究的目的:
- 扩大小麦FHB耐药性的遗传资源.
- 通过使用修改后的嵌套协会映射 (NAM) 面板,识别与FHB耐药性相关的新型和已知的遗传位点.
主要方法:
- 使用合成六倍体小麦 (SHW) 和硬红色春季小麦 (HRSW) 线路开发了一个修改后的NAM面板.
- 使用90K SNP阵列对面板进行基因定型,并在六个环境中评估FHB耐药性.
- 进行了全基因组关联研究 (GWAS) 以确定标记特征关联 (MTA).
主要成果:
- 确定了FHB耐药性的19个显著的MTA.
- 发现3个SNP携带来自SHW的抗性基因,2个来自HRSW的抗性基因和14个来自父母的抗性基因.
- 发现16个MTA与已知的FHB耐药性定量特征位点 (QTLs) 共定位,以及1D,2B和4A染色体上的3个MTA表明了新的耐药性位点.
结论:
- 这项研究确定了来自SHW背十字的有前途的耐药小麦品种.
- 发现了三个潜在的新型FHB耐药位点,以及十六个已知的位点.
- 这些发现为开发FHB耐药小麦品种提供了宝贵的遗传资源.
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